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FTase Substrate, Fluorogenic

FTase Substrate, Fluorogenic
Sequence (1LC):
Dansyl-GCVLS
Sequence (3LC):
Dansyl-Gly-Cys-Val-Leu-Ser
Catalog :
13684-01
Unit:
1 mgs
Price/Unit:
$963  (For other quantities please Contact Us or call 972-420-8505 (USA) 800-227-0627 (INTL.) or Fax 972.420.0442)
Purity:
>95%
Format:
Each vial contains lyophilized solid packaged under an inert gas and supplied as a trifluoroacetate salt
Description:

A pentapeptide substrate based on the carboxyl terminus of H-Ras with a dansyl group attached to the amino terminus. Serves as a substrate for continuous fluorescent monitoring of protein-farnesyl transferase (FTase) activity (kcat = 0.5 s-1; Km = 1.4 µM) in the presence of farnesyl diphosphate. S-farnesylation of the cysteine moiety results in approximately 13-fold enhancement in fluorescence intensity (monitored at l = 505 nm) accompanied by a shift in the emission maximum from 565 nm to 515 nm. Reported to be highly selective for FTase and is not recognized by geranylgeranyltransferase I. Useful for screening of potential FTase inhibitors.

Excitation max.: ~340 nm, Emission max.: ~505 nm e340 = 4,250 M-1 cm-1 

Note: PROTECT FROM LIGHT
Storage:
Upon receipt, store lyophilized peptide at -20°C or lower. Reconstituted peptide can be aliquoted and stored at -20 °C or lower.
Product Usage:
This product is for research use only. Not for use in diagnostic or therapeutic procedures.
Formula:
C31H46N6O9S2
M.W.:
710.9
Reference/Citations:

Reference:

  1. Pompliano, D.L., et al. 1992. J. Am. Chem. Soc. 114, 7945.
  2. Pompliano, D.L., et al. 1992. Biochemistry 31, 3800.
  3. Pickett, W.C., et al. 1995. Anal. Biochem. 225, 60.
  4. Hightower, K.E., et al. 1998. Biochemistry 37, 15555.
  5. Schlitzer, M., et al. 1999. Bioorg. Med. Chem. 7, 2037.
  6. Bohm, M., et al. 2001. J. Med. Chem. 44, 3117.