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Improving the stability of an RNA sequence

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11/01/2013

Improving the stability of an RNA sequence

Thioate groups can be incorporate in the RNA linkages between two bases. This approach is dependent on the specific application.
We recommend the incorporating of BNA monomers into synthetic oligonucleotides. BNA monomer contain a six member bridged structure with a unique N-O bond in the sugar moiety. This RNA analog was developed by Professor Emeritus Takeshi Imanishi of Osaka University. This moiety was designed to contain a nitrogen atom which is important in DNA chemistry as a conjugation site. It improves duplex and triplex stability by lowering the repulsion between negatively charged phosphate backbones.

These nucleic acid analogs can be easily incorporated into natural oligonucleotide strands. They provide flexibility in designing BNA/DNA and BNA/RNA hybrid oligonucleotides to satisfy the need for very high and sequence-specific hybridization with natural nucleic acids. Additionally, they possess a strong nuclease-resistant property. While first generation BNA (also known as LNA) are still used in various applications, Bio-Synthesis Inc. now offers third generation, six membered bridged 2', 4' BNANCs which have shown to possess superior properties to the earlier generation of locked nucleic acids and peptide nucleic acid.

BNA Advantages

* Improved hybridization selectivity and specificity
* Increased thermal duplex stability
* Enhanced allelic discrimination
* High biological stability to nuclease and protease
* Superior antisense inhibition and potency
* Flexible probe designs regardless of GC content
* Easily adaptable to many DNA or RNA detection system 

11/01/2013