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BNA FISH Probe for Chromosomal DNA Detection

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08/06/2012

BNA FISH Probe for Chromosomal DNA Detection

Fluorescence in-situ hybridization, or FISH, using specifically designed probes has become a widely used technique for many applications including cytogenetic diagnostics. However, the design of these probes is often time-consuming and may lack sensitivity and resolution. 
  
Bio-Synthesis's offers high affinity BNA enhanced FISH Probes (BFISH) that provides an alternative solution for a fast, sensitive and specific detection of specific DNA sequences such as chromsomal DNA.
 
We are happy to assist you with the design of BNA FISH probes.

Please contact us for more information.

You may also design your own BNA FISH probes using our guidelines.

! You design. We can Help !

 

Short guidelines for the design of BNA FISH probes

  • Detection probes are typically 20-25 nucleotides in length. However, shorter or longer probes can also be used.
  • Avoid stretches of 3 or more Gs or Cs. Avoid stretches of more than 4 BNA bases, except when very short (9-10 nt) oligonucleotides are designed.
  • Avoid self-complimentary BNAs since BNA oligonucleotides can hybridize very thightly with other BNA residues. 
  • Keep the GC-content between 30-60 %.
  • A Tm of approximately 75 °C is recommended.
  • Avoid the placement of BNA bases in palindrome sequences (G-C base pairs are more critical than A-T base pairs).
Other useful links:
  1. Can you suggest a BNA experimental condition for FISH application?
  2. Do you have suggested buffers for BNA experiment?
  3. Bridged Nucleic Acid Design Guidelines
  4. BNA for Duplex and Triplex Formation
  5. Allele Specific BNA Primer Design