The Ebola Virus Genome and Proteome

The Ebola virus is a single-stranded, negative-sense mini-genome RNA virus. One strain, the Zaire Ebola virus is responsible for the recent Ebola outbreak in West-Africa. Ebola viruses belong to the filoviridae family, and together with Paramyxoviridae, Rhabdoviridae, and Borna disease virus, Filoviridae viruses belong to the taxonomic order mononegavirales. The Ebola virus genome like all filovirdae genomes is similar to the Marburg virus genome. Filovirdae can cause severe hemorrhargic fever in non-human primates as well as in humans, to various degrees.

Mononegavirales is the term used for "nonsegmented negative-strand RNA viruses" (NNSV). These are enveloped viruses that have mini-genomes consisting of a single RNA molecule of negative or anti-mRNA sense. Hence the Ebola virus genome as well as all filovirus genomes are considered to be mini-genomes. A synthetic Ebola virus genome without is proteins appears to be non-infectious.

Nucleic acids isolated from negative strand RNA viruses or virus-infected cells cannot infect or initiate an infection cycle when introduced into the host cell. This criterion was used to distinguish “positive" from “negative”-strand RNA viruses. The viral genome needs to be first transcribed to produce mRNAs. Therefore, the purified virion RNA is not infectious. The virus needs to bring its own RNA polymerase into the cell in order to produce mRNA. To allow the virus to be infective a viral polymerase must be part of the viral particle or virion.

The use of non-infectious synthetic viral RNA allows for the design of PCR primers or probes as well as peptides and recombinant proteins for molecular diagnostics. Similarly, these molecules may lend themselves for the design and production of vaccines against the virus. 

A visual model of the ebola virus is availabel at the "Visual Science" science website:

 http://visual-science.com/projects/ebola/poster/.

Features of the unsegmented genome of negative-stranded RNA viruses are:

• Negative sense RNA in the virion
• Virion-associated RNA polymerase mediates transcription and replication
• Genome transcribed into 6-10 separate mRNAs from a single promoter
• Replication occurs by synthesis of a complete positive-sense RNA antigenome
• Nucleoprotein is the functional template for synthesis of replicative and mRNA
• Independently assembled nucleocapsids are enveloped at the cell surface at sites containing virus proteins
• Are mainly cytoplasmic
• Can occur in invertebrates, vertebrates and plants
  

Features of the family Filoviridae are:

• Filamentous forms with branching; sometimes U-shaped, 6-shaped or circular 
• Uniform diameter of 80 nm and varying lengths up to 14,000 nm. Infectious particle length is 790 nm for Marburg virus and 970 nm for Ebola virus
• Surface spikes of 10 nm length
• Helical nucleocapsid; 50 nm diameter, with an axial space of 20 nm diameter and helical periodicity of about 5 nm
• Filamentous with a linear ~13-19 kb mini-genome with a negative-sense single-stranded RNA of molecular weight (Mr) =  4.2 x 10⁶ 
• At least five (5) proteins; a large (polymerase) protein, a surface glycoprotein, two (2) nucleocapsid-associated proteins, and at least one other protein of unknown function
• Biology enigmatic; only two antigenically unrelated viruses known; blood borne infection of humans and monkeys

Filoviruses are responsible for newly emerging infections. Filoviruses are considered as Biosafety Level 4 agents, in comparison HIV is only considered as Biosafety Level 2+. Filoviruses can infect mice, hamsters, guinea pigs and monkeys. However, it is not known at presence where the virus originates in the wild. 

Most human epidemics appear to be blood-born spread, in hospitals often transmitted via contaminated needles, and transmitted via close contact with infected persons or their body fluids. Primary infections with Marburg and Ebola are usually 25 to 90% fatal.  Death is thought to occur because of visceral organ necrosis, for example of the liver, due to viral infection of tissue parenchymal cells. 

Viral RNA is not infectious by itself. Therefore, the use of cloned or synthetic viral RNA can be very useful for the development and production of diagnostic tests or the development of vaccines against filoviruses, for example, the Ebola virus. 

Research with the aim to develop a vaccine for Ebola has already been started for several years now. In 1998, the first immunization for Ebola virus infections that was successful was reported. 

“Abstract: Infection by Ebola virus causes rapidly progressive, often fatal, symptoms of fever, hemorrhage and hypotension. Previous attempts to elicit protective immunity for this disease have not met with success. We report here that protection against the lethal effects of Ebola virus can be achieved in an animal model by immunizing with plasmids encoding viral proteins. We analyzed immune responses to the viral nucleoprotein (NP) and the secreted or transmembrane forms of the glycoprotein (sGP or GP) and their ability to protect against infection in a guinea pig infection model analogous to the human disease. Protection was achieved and correlated with antibody titer and antigen-specific T-cell responses to sGP or GP. Immunity to Ebola virus can therefore be developed through genetic vaccination and may facilitate efforts to limit the spread of this disease.” 

{Xu L, Sanchez A, Yang Z, Zaki SR, Nabel EG, Nichol ST, Nabel GJ.; Immunization for Ebola virus infection. Nat Med. 1998 Jan;4(1):37-42.}

The result – a DNA vaccine encoding the glycoprotein (sGP or GP) of the Ebola virus evoked a T-cell based immune response in guinea pigs and protected the animals against infection. Further studies indicated that a DNA vaccine can is useful for vaccination. The use of DNA immunization together with adenovirus vectors encoding viral proteins in nonhuman primates resulted in the protection of crab-eating or cynomolgus macaques (Macaca fascicularis) from the lethal pathogen, the wild-type Zaire virus. 

“Abstract: Outbreaks of haemorrhagic fever caused by the Ebola virus are associated with high mortality rates that are a distinguishing feature of this human pathogen. The highest lethality is associated with the Zaire subtype, one of four strains identified to date. Its rapid progression allows little opportunity to develop natural immunity, and there is currently no effective anti-viral therapy. Therefore, vaccination offers a promising intervention to prevent infection and limit spread. Here we describe a highly effective vaccine strategy for Ebola virus infection in non-human primates. A combination of DNA immunization and boosting with adenoviral vectors that encode viral proteins generated cellular and humoral immunity in cynomolgus macaques. Challenge with a lethal dose of the highly pathogenic, wild-type, 1976 Mayinga strain of Ebola Zaire virus resulted in uniform infection in controls, who progressed to a moribund state and death in less than one week. In contrast, all vaccinated animals were asymptomatic for more than six months, with no detectable virus after the initial challenge. These findings demonstrate that it is possible to develop a preventive vaccine against Ebola virus infection in primates.”

 {Sullivan NJ, Sanchez A, Rollin PE, Yang ZY, Nabel GJ.; Development of a preventive vaccine for Ebola virus infection in primates. Nature. 2000 Nov 30;408(6812):605-9.}

Results from sequence analysis of Ebola viruses from outbreaks in 1976 and 1995 showed a high degree of genetic conservation for this virus type. An explanation of this could be that Ebola viruses may have coevolved with their natural host reservoirs and do not change a lot in the wild.

Reference

Biology of Negative Strand RNA Viruses: The Power of Reverse Genetics; Y. Kawaoka (Ed.). © Springer-Verlag Berlin Heidelberg 2004.

Ebihara H, Takada A, Kobasa D, Jones S, Neumann G, et al. (2006) Molecular determinants of Ebola virus virulence in mice. PLoS Pathog 2(7): e73. DOI: 10.1371/ journal.ppat.0020073.

MOLECULAR BASIS OF VIRUS EVOLUTION; Edited by ADRIAN J. GIBBS, CHARLES H. CALISHER, and FERNANDO GARCIA-ARENAL, © Cambridge University Press 1995.

http://www.ncbi.nlm.nih.gov/pubmed/?term=ebola+virus+review

 

The Zaire Ebola Virus Genome and Proteome

Graphical display and FASTA file from Pubmed.

Zaire ebolavirus isolate Ebola virus H.sapiens-tc/COD/1976/Yambuku-Mayinga, complete genome. NCBI Reference Sequence: NC_002549.1. 

Source

http://www.ncbi.nlm.nih.gov/nuccore/10313991?report=graph

 

 

LOCUS       NC_002549              18959 bp    cRNA    linear   VRL 27-AUG-2014

DEFINITION  Zaire ebolavirus isolate Ebola virus

            H.sapiens-tc/COD/1976/Yambuku-Mayinga, complete genome.

ACCESSION   NC_002549

VERSION     NC_002549.1  GI:10313991

DBLINK      BioProject: PRJNA14703

KEYWORDS    RefSeq.

SOURCE      Zaire ebolavirus (ZEBOV)

  ORGANISM  Zaire ebolavirus

            Viruses; ssRNA negative-strand viruses; Mononegavirales;

            Filoviridae; Ebolavirus.

REFERENCE   1  (bases 1 to 18959)

  AUTHORS   Volchkov,V.E., Volchkova,V.A., Chepurnov,A.A., Blinov,V.M.,

            Dolnik,O., Netesov,S.V. and Feldmann,H.

  TITLE     Characterization of the L gene and 5' trailer region of Ebola virus

  JOURNAL   J. Gen. Virol. 80 (Pt 2), 355-362 (1999)

   PUBMED   10073695

REFERENCE   2  (bases 1 to 18959)

  AUTHORS   Volchkov,V.E., Volchkova,V.A., Slenczka,W., Klenk,H.D. and

            Feldmann,H.

  TITLE     Release of viral glycoproteins during Ebola virus infection

  JOURNAL   Virology 245 (1), 110-119 (1998)

   PUBMED   9614872

REFERENCE   3  (bases 1 to 18959)

  AUTHORS   Volchkov,V.E., Feldmann,H., Volchkova,V.A. and Klenk,H.D.

  TITLE     Processing of the Ebola virus glycoprotein by the proprotein

            convertase furin

  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 95 (10), 5762-5767 (1998)

   PUBMED   9576958

REFERENCE   4  (bases 1 to 18959)

  AUTHORS   Volchkov,V.E., Becker,S., Volchkova,V.A., Ternovoj,V.A.,

            Kotov,A.N., Netesov,S.V. and Klenk,H.D.

  TITLE     GP mRNA of Ebola virus is edited by the Ebola virus polymerase and

            by T7 and vaccinia virus polymerases

  JOURNAL   Virology 214 (2), 421-430 (1995)

   PUBMED   8553543

REFERENCE   5  (bases 1 to 18959)

  AUTHORS   Bukreyev,A.A., Volchkov,V.E., Blinov,V.M. and Netesov,S.V.

  TITLE     The VP35 and VP40 proteins of filoviruses. Homology between Marburg

            and Ebola viruses

  JOURNAL   FEBS Lett. 322 (1), 41-46 (1993)

   PUBMED   8482365

REFERENCE   6  (bases 1 to 18959)

  CONSRTM   NCBI Genome Project

  TITLE     Direct Submission

  JOURNAL   Submitted (27-SEP-2000) National Center for Biotechnology

            Information, NIH, Bethesda, MD 20894, USA

REFERENCE   7  (bases 1 to 18959)

  AUTHORS   Volchkov,V.E.

  TITLE     Direct Submission

  JOURNAL   Submitted (02-JUN-2000) Institute of Virology, Philipps-University

            Marburg, Robert-Koch-Str. 17, Marburg 35037, Germany

  REMARK    Sequence update by submitter

REFERENCE   8  (bases 1 to 18959)

  AUTHORS   Volchkov,V.E.

  TITLE     Direct Submission

  JOURNAL   Submitted (20-AUG-1998) Institute of Virology, Philipps-University

            Marburg, Robert-Koch-Str. 17, Marburg 35037, Germany

COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final

            NCBI review. The reference sequence is identical to AF086833.

            COMPLETENESS: full length.

FEATURES             Location/Qualifiers

     source          1..18959

                     /organism="Zaire ebolavirus"

                     /mol_type="viral cRNA"

                     /isolate="Ebola virus

                     H.sapiens-tc/COD/1976/Yambuku-Mayinga"

                     /db_xref="taxon:186538"

     5'UTR           1..55

                     /note="putative leader region"

                     /citation=[1]

                     /function="regulation or initiation of RNA replication"

     gene            56..3026

                     /gene="NP"

                     /locus_tag="ZEBOVgp1"

                     /db_xref="GeneID:911830"

     mRNA            56..3026

                     /gene="NP"

                     /locus_tag="ZEBOVgp1"

                     /product="nucleoprotein"

                     /db_xref="GeneID:911830"

     misc_signal     56..67

                     /gene="NP"

                     /locus_tag="ZEBOVgp1"

                     /note="putative; transcription start signal"

                     /citation=[1]

     CDS             470..2689

                     /gene="NP"

                     /locus_tag="ZEBOVgp1"

                     /function="encapsidation of genomic RNA"

                     /codon_start=1

                     /product="nucleoprotein"

                     /protein_id="NP_066243.1"

                     /db_xref="GI:10314000"

                     /db_xref="GeneID:911830"

                     /translation="MDSRPQKIWMAPSLTESDMDYHKILTAGLSVQQGIVRQRVIPVY

                     QVNNLEEICQLIIQAFEAGVDFQESADSFLLMLCLHHAYQGDYKLFLESGAVKYLEGH

                     GFRFEVKKRDGVKRLEELLPAVSSGKNIKRTLAAMPEEETTEANAGQFLSFASLFLPK

                     LVVGEKACLEKVQRQIQVHAEQGLIQYPTAWQSVGHMMVIFRLMRTNFLIKFLLIHQG

                     MHMVAGHDANDAVISNSVAQARFSGLLIVKTVLDHILQKTERGVRLHPLARTAKVKNE

                     VNSFKAALSSLAKHGEYAPFARLLNLSGVNNLEHGLFPQLSAIALGVATAHGSTLAGV

                     NVGEQYQQLREAATEAEKQLQQYAESRELDHLGLDDQEKKILMNFHQKKNEISFQQTN

                     AMVTLRKERLAKLTEAITAASLPKTSGHYDDDDDIPFPGPINDDDNPGHQDDDPTDSQ

                     DTTIPDVVVDPDDGSYGEYQSYSENGMNAPDDLVLFDLDEDDEDTKPVPNRSTKGGQQ

                     KNSQKGQHIEGRQTQSRPIQNVPGPHRTIHHASAPLTDNDRRNEPSGSTSPRMLTPIN

                     EEADPLDDADDETSSLPPLESDDEEQDRDGTSNRTPTVAPPAPVYRDHSEKKELPQDE

                     QQDQDHTQEARNQDSDNTQSEHSFEEMYRHILRSQGPFDAVLYYHMMKDEPVVFSTSD

                     GKEYTYPDSLEEEYPPWLTEKEAMNEENRFVTLDGQQFYWPVMNHKNKFMAILQHHQ"

     misc_feature    524..2671

                     /gene="NP"

                     /locus_tag="ZEBOVgp1"

                     /note="Ebola nucleoprotein; Region: Ebola_NP; pfam05505"

                     /db_xref="CDD:147601"

     polyA_signal    3015..3026

                     /gene="NP"

                     /locus_tag="ZEBOVgp1"

     misc_feature    3027..3031

                     /note="intergenic region"

     gene            3032..4407

                     /gene="VP35"

                     /locus_tag="ZEBOVgp2"

                     /db_xref="GeneID:911827"

     mRNA            3032..4407

                     /gene="VP35"

                     /locus_tag="ZEBOVgp2"

                     /product="VP35"

                     /citation=[5]

                     /db_xref="GeneID:911827"

     misc_signal     3032..3043

                     /gene="VP35"

                     /locus_tag="ZEBOVgp2"

                     /note="putative; transcription start signal"

                     /citation=[5]

     CDS             3129..4151

                     /gene="VP35"

                     /locus_tag="ZEBOVgp2"

                     /function="polymerase complex protein"

                     /citation=[5]

                     /codon_start=1

                     /product="polymerase complex protein"

                     /protein_id="NP_066244.1"

                     /db_xref="GI:10313992"

                     /db_xref="GeneID:911827"

                     /translation="MTTRTKGRGHTAATTQNDRMPGPELSGWISEQLMTGRIPVSDIF

                     CDIENNPGLCYASQMQQTKPNPKTRNSQTQTDPICNHSFEEVVQTLASLATVVQQQTI

                     ASESLEQRITSLENGLKPVYDMAKTISSLNRVCAEMVAKYDLLVMTTGRATATAAATE

                     AYWAEHGQPPPGPSLYEESAIRGKIESRDETVPQSVREAFNNLNSTTSLTEENFGKPD

                     ISAKDLRNIMYDHLPGFGTAFHQLVQVICKLGKDSNSLDIIHAEFQASLAEGDSPQCA

                     LIQITKRVPIFQDAAPPVIHIRSRGDIPRACQKSLRPVPPSPKIDRGWVCVFQLQDGK

                     TLGLKI"

     misc_feature    3186..4148

                     /gene="VP35"

                     /locus_tag="ZEBOVgp2"

                     /note="Filoviridae VP35; Region: Filo_VP35; pfam02097"

                     /db_xref="CDD:145320"

     gene            4390..5894

                     /gene="VP40"

                     /locus_tag="ZEBOVgp3"

                     /db_xref="GeneID:911825"

     mRNA            4390..5894

                     /gene="VP40"

                     /locus_tag="ZEBOVgp3"

                     /product="VP40"

                     /citation=[5]

                     /db_xref="GeneID:911825"

     misc_signal     4390..4401

                     /gene="VP40"

                     /locus_tag="ZEBOVgp3"

                     /note="transcription start signal"

                     /citation=[5]

     polyA_signal    4397..4407

                     /gene="VP35"

                     /locus_tag="ZEBOVgp2"

                     /citation=[5]

     CDS             4479..5459

                     /gene="VP40"

                     /locus_tag="ZEBOVgp3"

                     /citation=[5]

                     /codon_start=1

                     /product="matrix protein"

                     /protein_id="NP_066245.1"

                     /db_xref="GI:10313993"

                     /db_xref="GeneID:911825"

                     /translation="MRRVILPTAPPEYMEAIYPVRSNSTIARGGNSNTGFLTPESVNG

                     DTPSNPLRPIADDTIDHASHTPGSVSSAFILEAMVNVISGPKVLMKQIPIWLPLGVAD

                     QKTYSFDSTTAAIMLASYTITHFGKATNPLVRVNRLGPGIPDHPLRLLRIGNQAFLQE

                     FVLPPVQLPQYFTFDLTALKLITQPLPAATWTDDTPTGSNGALRPGISFHPKLRPILL

                     PNKSGKKGNSADLTSPEKIQAIMTSLQDFKIVPIDPTKNIMGIEVPETLVHKLTGKKV

                     TSKNGQPIIPVLLPKYIGLDPVAPGDLTMVITQDCDTCHSPASLPAVIEK"

     misc_feature    4479..5363

                     /gene="VP40"

                     /locus_tag="ZEBOVgp3"

                     /note="Matrix protein VP40; Region: VP40; pfam07447"

                     /db_xref="CDD:116068"

     polyA_signal    5883..5894

                     /gene="VP40"

                     /locus_tag="ZEBOVgp3"

                     /citation=[5]

     misc_feature    5895..5899

                     /note="intergenic region"

     gene            5900..8305

                     /gene="GP"

                     /locus_tag="ZEBOVgp4"

                     /db_xref="GeneID:911829"

     mRNA            5900..8305

                     /gene="GP"

                     /locus_tag="ZEBOVgp4"

                     /product="sGP"

                     /note="unedited mRNA"

                     /citation=[4]

                     /db_xref="GeneID:911829"

     misc_signal     5900..5911

                     /gene="GP"

                     /locus_tag="ZEBOVgp4"

                     /note="putative; transcription start signal"

                     /citation=[4]

     CDS             join(6039..6923,6923..8068)

                     /gene="GP"

                     /locus_tag="ZEBOVgp4"

                     /function="receptor binding and fusion"

                     /artificial_location="low-quality sequence region"

                     /note="virion spike glycoprotein precursor; an addition A

                     residue is inserted during transcription; encodes two

                     disulfide linked subunits GP1 and GP2"

                     /citation=[2]

                     /citation=[3]

                     /citation=[4]

                     /codon_start=1

                     /product="spike glycoprotein"

                     /protein_id="NP_066246.1"

                     /db_xref="GI:10313995"

                     /db_xref="GeneID:911829"

                     /translation="MGVTGILQLPRDRFKRTSFFLWVIILFQRTFSIPLGVIHNSTLQ

                     VSDVDKLVCRDKLSSTNQLRSVGLNLEGNGVATDVPSATKRWGFRSGVPPKVVNYEAG

                     EWAENCYNLEIKKPDGSECLPAAPDGIRGFPRCRYVHKVSGTGPCAGDFAFHKEGAFF

                     LYDRLASTVIYRGTTFAEGVVAFLILPQAKKDFFSSHPLREPVNATEDPSSGYYSTTI

                     RYQATGFGTNETEYLFEVDNLTYVQLESRFTPQFLLQLNETIYTSGKRSNTTGKLIWK

                     VNPEIDTTIGEWAFWETKKNLTRKIRSEELSFTVVSNGAKNISGQSPARTSSDPGTNT

                     TTEDHKIMASENSSAMVQVHSQGREAAVSHLTTLATISTSPQSLTTKPGPDNSTHNTP

                     VYKLDISEATQVEQHHRRTDNDSTASDTPSATTAAGPPKAENTNTSKSTDFLDPATTT

                     SPQNHSETAGNNNTHHQDTGEESASSGKLGLITNTIAGVAGLITGGRRTRREAIVNAQ

                     PKCNPNLHYWTTQDEGAAIGLAWIPYFGPAAEGIYIEGLMHNQDGLICGLRQLANETT

                     QALQLFLRATTELRTFSILNRKAIDFLLQRWGGTCHILGPDCCIEPHDWTKNITDKID

                     QIIHDFVDKTLPDQGDNDNWWTGWRQWIPAGIGVTGVIIAVIALFCICKFVF"

     misc_feature    7529..7540

                     /gene="GP"

                     /locus_tag="ZEBOVgp4"

                     /note="encodes the glycoprotein cleavage site, precursor

                     GP is cleaved by subtilisin-like cellular protease furin

                     into subunits GP1 and GP2 that are linked by a disulfide

                     bond"

                     /citation=[3]

     misc_feature    7793..7870

                     /gene="GP"

                     /locus_tag="ZEBOVgp4"

                     /note="immunosuppressive motif; other site"

     misc_feature    7988..8053

                     /gene="GP"

                     /locus_tag="ZEBOVgp4"

                     /note="transmembrane anchor; transmembrane region"

     misc_feature    7706..7924

                     /gene="GP"

                     /locus_tag="ZEBOVgp4"

                     /note="heptad repeat 1-heptad repeat 2 region of the

                     transmembrane subunit of Filoviridae viruses, Ebola virus

                     and Marburg virus, and related domains; Region:

                     Ebola-like_HR1-HR2; cd09850"

                     /db_xref="CDD:197367"

     misc_feature    join(6081..6923,6923..7153)

                     /gene="GP"

                     /locus_tag="ZEBOVgp4"

                     /note="Filovirus glycoprotein; Region: Filo_glycop;

                     pfam01611"

                     /db_xref="CDD:110602"

     misc_feature    7706..7732

                     /gene="GP"

                     /locus_tag="ZEBOVgp4"

                     /note="HR1A; other site"

                     /db_xref="CDD:197367"

     misc_feature    7733..7762

                     /gene="GP"

                     /locus_tag="ZEBOVgp4"

                     /note="HR1B; other site"

                     /db_xref="CDD:197367"

     misc_feature    7763..7783

                     /gene="GP"

                     /locus_tag="ZEBOVgp4"

                     /note="HR1C; other site"

                     /db_xref="CDD:197367"

     misc_feature    7784..7831

                     /gene="GP"

                     /locus_tag="ZEBOVgp4"

                     /note="HR1D; other site"

                     /db_xref="CDD:197367"

     misc_feature    7787..7837

                     /gene="GP"

                     /locus_tag="ZEBOVgp4"

                     /note="immunosuppressive region; other site"

                     /db_xref="CDD:197367"

     misc_feature    order(7838..7858,7859..7861)

                     /gene="GP"

                     /locus_tag="ZEBOVgp4"

                     /note="CX(6,7)C motif; other site"

                     /db_xref="CDD:197367"

     misc_feature    7886..7924

                     /gene="GP"

                     /locus_tag="ZEBOVgp4"

                     /note="HR2; other site"

                     /db_xref="CDD:197367"

     misc_feature    order(7784..7786,7793..7795)

                     /gene="GP"

                     /locus_tag="ZEBOVgp4"

                     /note="Cl binding site [ion binding]; other site"

                     /db_xref="CDD:197367"

     misc_feature    order(7706..7714,7718..7723,7727..7732,7736..7744,

                     7748..7756,7760..7765,7769..7777,7781..7807,7811..7819,

                     7823..7828,7844..7849,7856..7858,7865..7876,7880..7882,

                     7889..7894,7901..7903,7910..7915,7922..7924)

                     /gene="GP"

                     /locus_tag="ZEBOVgp4"

                     /note="homotrimer interface [polypeptide binding]; other

                     site"

                     /db_xref="CDD:197367"

     misc_feature    order(7706..7714,7718..7726,7730..7735,7739..7747,

                     7754..7768,7772..7783,7787..7792,7796..7804,7808..7813,

                     7817..7819)

                     /gene="GP"

                     /locus_tag="ZEBOVgp4"

                     /note="HR1-GP1 interface [polypeptide binding]; other

                     site"

                     /db_xref="CDD:197367"

     CDS             6039..7133

                     /gene="GP"

                     /locus_tag="ZEBOVgp4"

                     /note="sGP, small non-structural, secreted glycoprotein;

                     sGP secreted as a anti-parallel oriented homodimer"

                     /citation=[4]

                     /codon_start=1

                     /product="small secreted glycoprotein"

                     /protein_id="NP_066247.1"

                     /db_xref="GI:10313994"

                     /db_xref="GeneID:911829"

                     /translation="MGVTGILQLPRDRFKRTSFFLWVIILFQRTFSIPLGVIHNSTLQ

                     VSDVDKLVCRDKLSSTNQLRSVGLNLEGNGVATDVPSATKRWGFRSGVPPKVVNYEAG

                     EWAENCYNLEIKKPDGSECLPAAPDGIRGFPRCRYVHKVSGTGPCAGDFAFHKEGAFF

                     LYDRLASTVIYRGTTFAEGVVAFLILPQAKKDFFSSHPLREPVNATEDPSSGYYSTTI

                     RYQATGFGTNETEYLFEVDNLTYVQLESRFTPQFLLQLNETIYTSGKRSNTTGKLIWK

                     VNPEIDTTIGEWAFWETKKTSLEKFAVKSCLSQLYQTEPKTSVVRVRRELLPTQGPTQ

                     QLKTTKSWLQKIPLQWFKCTVKEGKLQCRI"

     misc_feature    6081..7130

                     /gene="GP"

                     /locus_tag="ZEBOVgp4"

                     /note="Filovirus glycoprotein; Region: Filo_glycop;

                     pfam01611"

                     /db_xref="CDD:110602"

     CDS             join(6039..6922,6924..6933)

                     /gene="GP"

                     /locus_tag="ZEBOVgp4"

                     /artificial_location="low-quality sequence region"

                     /note="ssGP; second non-structural secreted glycoprotein;

                     secreted in a monomeric form; one A residue is deleted or

                     two additional A residues are inserted at the editing site

                     during transcription of the GP gene"

                     /citation=[4]

                     /codon_start=1

                     /product="second secreted glycoprotein"

                     /protein_id="NP_066248.1"

                     /db_xref="GI:10313996"

                     /db_xref="GeneID:911829"

                     /translation="MGVTGILQLPRDRFKRTSFFLWVIILFQRTFSIPLGVIHNSTLQ

                     VSDVDKLVCRDKLSSTNQLRSVGLNLEGNGVATDVPSATKRWGFRSGVPPKVVNYEAG

                     EWAENCYNLEIKKPDGSECLPAAPDGIRGFPRCRYVHKVSGTGPCAGDFAFHKEGAFF

                     LYDRLASTVIYRGTTFAEGVVAFLILPQAKKDFFSSHPLREPVNATEDPSSGYYSTTI

                     RYQATGFGTNETEYLFEVDNLTYVQLESRFTPQFLLQLNETIYTSGKRSNTTGKLIWK

                     VNPEIDTTIGEWAFWETKKPH"

     misc_feature    join(6081..6922,6924..>6924)

                     /gene="GP"

                     /locus_tag="ZEBOVgp4"

                     /note="Filovirus glycoprotein; Region: Filo_glycop;

                     pfam01611"

                     /db_xref="CDD:110602"

     misc_signal     6918..6924

                     /gene="GP"

                     /locus_tag="ZEBOVgp4"

                     /note="additional A residues are inserted or deleted

                     during transcription of the GP gene by the viral

                     polymerase"

                     /citation=[4]

                     /function="RNA editing"

     gene            8288..9740

                     /gene="VP30"

                     /locus_tag="ZEBOVgp5"

                     /db_xref="GeneID:911826"

     mRNA            8288..9740

                     /gene="VP30"

                     /locus_tag="ZEBOVgp5"

                     /product="VP30"

                     /db_xref="GeneID:911826"

     misc_signal     8288..8299

                     /gene="VP30"

                     /locus_tag="ZEBOVgp5"

                     /note="putative; transcription start signal"

     polyA_signal    8295..8305

                     /gene="GP"

                     /locus_tag="ZEBOVgp4"

                     /citation=[4]

     CDS             8509..9375

                     /gene="VP30"

                     /locus_tag="ZEBOVgp5"

                     /note="polymerase complex protein"

                     /codon_start=1

                     /product="minor nucleoprotein"

                     /protein_id="NP_066249.1"

                     /db_xref="GI:10313997"

                     /db_xref="GeneID:911826"

                     /translation="MEASYERGRPRAARQHSRDGHDHHVRARSSSRENYRGEYRQSRS

                     ASQVRVPTVFHKKRVEPLTVPPAPKDICPTLKKGFLCDSSFCKKDHQLESLTDRELLL

                     LIARKTCGSVEQQLNITAPKDSRLANPTADDFQQEEGPKITLLTLIKTAEHWARQDIR

                     TIEDSKLRALLTLCAVMTRKFSKSQLSLLCETHLRREGLGQDQAEPVLEVYQRLHSDK

                     GGSFEAALWQQWDRQSLIMFITAFLNIALQLPCESSAVVVSGLRTLVPQSDNEEASTN

                     PGTCSWSDEGTP"

     misc_feature    8932..9321

                     /gene="VP30"

                     /locus_tag="ZEBOVgp5"

                     /note="Ebola virus-specific transcription factor VP30;

                     Region: Transcript_VP30; pfam11507"

                     /db_xref="CDD:151944"

     polyA_signal    9730..9740

                     /gene="VP30"

                     /locus_tag="ZEBOVgp5"

                     /note="putative"

     misc_feature    9741..9884

                     /note="intergenic region"

     gene            9885..11518

                     /gene="VP24"

                     /locus_tag="ZEBOVgp6"

                     /note="putative"

                     /db_xref="GeneID:911828"

     mRNA            9885..11496

                     /gene="VP24"

                     /locus_tag="ZEBOVgp6"

                     /product="VP24"

                     /db_xref="GeneID:911828"

     misc_signal     9885..9896

                     /gene="VP24"

                     /locus_tag="ZEBOVgp6"

                     /note="transcription start signal"

     CDS             10345..11100

                     /gene="VP24"

                     /locus_tag="ZEBOVgp6"

                     /codon_start=1

                     /product="membrane-associated protein"

                     /protein_id="NP_066250.1"

                     /db_xref="GI:10313998"

                     /db_xref="GeneID:911828"

                     /translation="MAKATGRYNLISPKKDLEKGVVLSDLCNFLVSQTIQGWKVYWAG

                     IEFDVTHKGMALLHRLKTNDFAPAWSMTRNLFPHLFQNPNSTIESPLWALRVILAAGI

                     QDQLIDQSLIEPLAGALGLISDWLLTTNTNHFNMRTQRVKEQLSLKMLSLIRSNILKF

                     INKLDALHVVNYNGLLSSIEIGTQNHTIIITRTNMGFLVELQEPDKSAMNRMKPGPAK

                     FSLLHESTLKAFTQGSSTRMQSLILEFNSSLAI"

     misc_feature    10369..11040

                     /gene="VP24"

                     /locus_tag="ZEBOVgp6"

                     /note="Filovirus membrane-associated protein VP24; Region:

                     Filo_VP24; pfam06389"

                     /db_xref="CDD:253701"

     polyA_signal    11485..11496

                     /gene="VP24"

                     /locus_tag="ZEBOVgp6"

                     /note="putative"

     misc_feature    11497..11500

                     /gene="VP24"

                     /locus_tag="ZEBOVgp6"

                     /note="intergenic region"

     gene            11501..18282

                     /gene="L"

                     /locus_tag="ZEBOVgp7"

                     /db_xref="GeneID:911824"

     mRNA            11501..18282

                     /gene="L"

                     /locus_tag="ZEBOVgp7"

                     /product="polymerase"

                     /citation=[1]

                     /db_xref="GeneID:911824"

     misc_signal     11501..11512

                     /gene="L"

                     /locus_tag="ZEBOVgp7"

                     /note="transcription start signal"

                     /citation=[1]

     polyA_signal    11508..11518

                     /gene="VP24"

                     /locus_tag="ZEBOVgp6"

     CDS             11581..18219

                     /gene="L"

                     /locus_tag="ZEBOVgp7"

                     /function="synthesis of viral RNAs; transcriptional RNA

                     editing"

                     /note="polymerase"

                     /citation=[1]

                     /codon_start=1

                     /product="RNA-dependent RNA polymerase"

                     /protein_id="NP_066251.1"

                     /db_xref="GI:10313999"

                     /db_xref="GeneID:911824"

                     /translation="MATQHTQYPDARLSSPIVLDQCDLVTRACGLYSSYSLNPQLRNC

                     KLPKHIYRLKYDVTVTKFLSDVPVATLPIDFIVPVLLKALSGNGFCPVEPRCQQFLDE

                     IIKYTMQDALFLKYYLKNVGAQEDCVDEHFQEKILSSIQGNEFLHQMFFWYDLAILTR

                     RGRLNRGNSRSTWFVHDDLIDILGYGDYVFWKIPISMLPLNTQGIPHAAMDWYQASVF

                     KEAVQGHTHIVSVSTADVLIMCKDLITCRFNTTLISKIAEIEDPVCSDYPNFKIVSML

                     YQSGDYLLSILGSDGYKIIKFLEPLCLAKIQLCSKYTERKGRFLTQMHLAVNHTLEEI

                     TEMRALKPSQAQKIREFHRTLIRLEMTPQQLCELFSIQKHWGHPVLHSETAIQKVKKH

                     ATVLKALRPIVIFETYCVFKYSIAKHYFDSQGSWYSVTSDRNLTPGLNSYIKRNQFPP

                     LPMIKELLWEFYHLDHPPLFSTKIISDLSIFIKDRATAVERTCWDAVFEPNVLGYNPP

                     HKFSTKRVPEQFLEQENFSIENVLSYAQKLEYLLPQYRNFSFSLKEKELNVGRTFGKL

                     PYPTRNVQTLCEALLADGLAKAFPSNMMVVTEREQKESLLHQASWHHTSDDFGEHATV

                     RGSSFVTDLEKYNLAFRYEFTAPFIEYCNRCYGVKNVFNWMHYTIPQCYMHVSDYYNP

                     PHNLTLENRDNPPEGPSSYRGHMGGIEGLQQKLWTSISCAQISLVEIKTGFKLRSAVM

                     GDNQCITVLSVFPLETDADEQEQSAEDNAARVAASLAKVTSACGIFLKPDETFVHSGF

                     IYFGKKQYLNGVQLPQSLKTATRMAPLSDAIFDDLQGTLASIGTAFERSISETRHIFP

                     CRITAAFHTFFSVRILQYHHLGFNKGFDLGQLTLGKPLDFGTISLALAVPQVLGGLSF

                     LNPEKCFYRNLGDPVTSGLFQLKTYLRMIEMDDLFLPLIAKNPGNCTAIDFVLNPSGL

                     NVPGSQDLTSFLRQIVRRTITLSAKNKLINTLFHASADFEDEMVCKWLLSSTPVMSRF

                     AADIFSRTPSGKRLQILGYLEGTRTLLASKIINNNTETPVLDRLRKITLQRWSLWFSY

                     LDHCDNILAEALTQITCTVDLAQILREYSWAHILEGRPLIGATLPCMIEQFKVFWLKP

                     YEQCPQCSNAKQPGGKPFVSVAVKKHIVSAWPNASRISWTIGDGIPYIGSRTEDKIGQ

                     PAIKPKCPSAALREAIELASRLTWVTQGSSNSDLLIKPFLEARVNLSVQEILQMTPSH

                     YSGNIVHRYNDQYSPHSFMANRMSNSATRLIVSTNTLGEFSGGGQSARDSNIIFQNVI

                     NYAVALFDIKFRNTEATDIQYNRAHLHLTKCCTREVPAQYLTYTSTLDLDLTRYRENE

                     LIYDSNPLKGGLNCNISFDNPFFQGKRLNIIEDDLIRLPHLSGWELAKTIMQSIISDS

                     NNSSTDPISSGETRSFTTHFLTYPKIGLLYSFGAFVSYYLGNTILRTKKLTLDNFLYY

                     LTTQIHNLPHRSLRILKPTFKHASVMSRLMSIDPHFSIYIGGAAGDRGLSDAARLFLR

                     TSISSFLTFVKEWIINRGTIVPLWIVYPLEGQNPTPVNNFLYQIVELLVHDSSRQQAF

                     KTTISDHVHPHDNLVYTCKSTASNFFHASLAYWRSRHRNSNRKYLARDSSTGSSTNNS

                     DGHIERSQEQTTRDPHDGTERNLVLQMSHEIKRTTIPQENTHQGPSFQSFLSDSACGT

                     ANPKLNFDRSRHNVKFQDHNSASKREGHQIISHRLVLPFFTLSQGTRQLTSSNESQTQ

                     DEISKYLRQLRSVIDTTVYCRFTGIVSSMHYKLDEVLWEIESFKSAVTLAEGEGAGAL

                     LLIQKYQVKTLFFNTLATESSIESEIVSGMTTPRMLLPVMSKFHNDQIEIILNNSASQ

                     ITDITNPTWFKDQRARLPKQVEVITMDAETTENINRSKLYEAVYKLILHHIDPSVLKA

                     VVLKVFLSDTEGMLWLNDNLAPFFATGYLIKPITSSARSSEWYLCLTNFLSTTRKMPH

                     QNHLSCKQVILTALQLQIQRSPYWLSHLTQYADCELHLSYIRLGFPSLEKVLYHRYNL

                     VDSKRGPLVSITQHLAHLRAEIRELTNDYNQQRQSRTQTYHFIRTAKGRITKLVNDYL

                     KFFLIVQALKHNGTWQAEFKKLPELISVCNRFYHIRDCNCEERFLVQTLYLHRMQDSE

                     VKLIERLTGLLSLFPDGLYRFD"

     misc_feature    11608..14853

                     /gene="L"

                     /locus_tag="ZEBOVgp7"

                     /note="Mononegavirales RNA dependent RNA polymerase;

                     Region: Mononeg_RNA_pol; pfam00946"

                     /db_xref="CDD:250248"

     misc_feature    15223..18192

                     /gene="L"

                     /locus_tag="ZEBOVgp7"

                     /note="mRNA capping enzyme, paramyxovirus family; Region:

                     paramyx_RNAcap; TIGR04198"

                     /db_xref="CDD:234496"

     polyA_signal    18272..18282

                     /gene="L"

                     /locus_tag="ZEBOVgp7"

                     /citation=[1]

     3'UTR           18283..18959

                     /note="putative trailer region"

                     /citation=[1]

                     /function="regulation or initiation of RNA replication"

ORIGIN      

        1 cggacacaca aaaagaaaga agaattttta ggatcttttg tgtgcgaata actatgagga

       61 agattaataa ttttcctctc attgaaattt atatcggaat ttaaattgaa attgttactg

      121 taatcacacc tggtttgttt cagagccaca tcacaaagat agagaacaac ctaggtctcc

      181 gaagggagca agggcatcag tgtgctcagt tgaaaatccc ttgtcaacac ctaggtctta

      241 tcacatcaca agttccacct cagactctgc agggtgatcc aacaacctta atagaaacat

      301 tattgttaaa ggacagcatt agttcacagt caaacaagca agattgagaa ttaaccttgg

      361 ttttgaactt gaacacttag gggattgaag attcaacaac cctaaagctt ggggtaaaac

      421 attggaaata gttaaaagac aaattgctcg gaatcacaaa attccgagta tggattctcg

      481 tcctcagaaa atctggatgg cgccgagtct cactgaatct gacatggatt accacaagat

      541 cttgacagca ggtctgtccg ttcaacaggg gattgttcgg caaagagtca tcccagtgta

      601 tcaagtaaac aatcttgaag aaatttgcca acttatcata caggcctttg aagcaggtgt

      661 tgattttcaa gagagtgcgg acagtttcct tctcatgctt tgtcttcatc atgcgtacca

      721 gggagattac aaacttttct tggaaagtgg cgcagtcaag tatttggaag ggcacgggtt

      781 ccgttttgaa gtcaagaagc gtgatggagt gaagcgcctt gaggaattgc tgccagcagt

      841 atctagtgga aaaaacatta agagaacact tgctgccatg ccggaagagg agacaactga

      901 agctaatgcc ggtcagtttc tctcctttgc aagtctattc cttccgaaat tggtagtagg

      961 agaaaaggct tgccttgaga aggttcaaag gcaaattcaa gtacatgcag agcaaggact

     1021 gatacaatat ccaacagctt ggcaatcagt aggacacatg atggtgattt tccgtttgat

     1081 gcgaacaaat tttctgatca aatttctcct aatacaccaa gggatgcaca tggttgccgg

     1141 gcatgatgcc aacgatgctg tgatttcaaa ttcagtggct caagctcgtt tttcaggctt

     1201 attgattgtc aaaacagtac ttgatcatat cctacaaaag acagaacgag gagttcgtct

     1261 ccatcctctt gcaaggaccg ccaaggtaaa aaatgaggtg aactccttta aggctgcact

     1321 cagctccctg gccaagcatg gagagtatgc tcctttcgcc cgacttttga acctttctgg

     1381 agtaaataat cttgagcatg gtcttttccc tcaactatcg gcaattgcac tcggagtcgc

     1441 cacagcacac gggagtaccc tcgcaggagt aaatgttgga gaacagtatc aacaactcag

     1501 agaggctgcc actgaggctg agaagcaact ccaacaatat gcagagtctc gcgaacttga

     1561 ccatcttgga cttgatgatc aggaaaagaa aattcttatg aacttccatc agaaaaagaa

     1621 cgaaatcagc ttccagcaaa caaacgctat ggtaactcta agaaaagagc gcctggccaa

     1681 gctgacagaa gctatcactg ctgcgtcact gcccaaaaca agtggacatt acgatgatga

     1741 tgacgacatt ccctttccag gacccatcaa tgatgacgac aatcctggcc atcaagatga

     1801 tgatccgact gactcacagg atacgaccat tcccgatgtg gtggttgatc ccgatgatgg

     1861 aagctacggc gaataccaga gttactcgga aaacggcatg aatgcaccag atgacttggt

     1921 cctattcgat ctagacgagg acgacgagga cactaagcca gtgcctaata gatcgaccaa

     1981 gggtggacaa cagaagaaca gtcaaaaggg ccagcatata gagggcagac agacacaatc

     2041 caggccaatt caaaatgtcc caggccctca cagaacaatc caccacgcca gtgcgccact

     2101 cacggacaat gacagaagaa atgaaccctc cggctcaacc agccctcgca tgctgacacc

     2161 aattaacgaa gaggcagacc cactggacga tgccgacgac gagacgtcta gccttccgcc

     2221 cttggagtca gatgatgaag agcaggacag ggacggaact tccaaccgca cacccactgt

     2281 cgccccaccg gctcccgtat acagagatca ctctgaaaag aaagaactcc cgcaagacga

     2341 gcaacaagat caggaccaca ctcaagaggc caggaaccag gacagtgaca acacccagtc

     2401 agaacactct tttgaggaga tgtatcgcca cattctaaga tcacaggggc catttgatgc

     2461 tgttttgtat tatcatatga tgaaggatga gcctgtagtt ttcagtacca gtgatggcaa

     2521 agagtacacg tatccagact cccttgaaga ggaatatcca ccatggctca ctgaaaaaga

     2581 ggctatgaat gaagagaata gatttgttac attggatggt caacaatttt attggccggt

     2641 gatgaatcac aagaataaat tcatggcaat cctgcaacat catcagtgaa tgagcatgga

     2701 acaatgggat gattcaaccg acaaatagct aacattaagt agtcaaggaa cgaaaacagg

     2761 aagaattttt gatgtctaag gtgtgaatta ttatcacaat aaaagtgatt cttatttttg

     2821 aatttaaagc tagcttatta ttactagccg tttttcaaag ttcaatttga gtcttaatgc

     2881 aaataggcgt taagccacag ttatagccat aattgtaact caatattcta actagcgatt

     2941 tatctaaatt aaattacatt atgcttttat aacttaccta ctagcctgcc caacatttac

     3001 acgatcgttt tataattaag aaaaaactaa tgatgaagat taaaaccttc atcatcctta

     3061 cgtcaattga attctctagc actcgaagct tattgtcttc aatgtaaaag aaaagctggt

     3121 ctaacaagat gacaactaga acaaagggca ggggccatac tgcggccacg actcaaaacg

     3181 acagaatgcc aggccctgag ctttcgggct ggatctctga gcagctaatg accggaagaa

     3241 ttcctgtaag cgacatcttc tgtgatattg agaacaatcc aggattatgc tacgcatccc

     3301 aaatgcaaca aacgaagcca aacccgaaga cgcgcaacag tcaaacccaa acggacccaa

     3361 tttgcaatca tagttttgag gaggtagtac aaacattggc ttcattggct actgttgtgc

     3421 aacaacaaac catcgcatca gaatcattag aacaacgcat tacgagtctt gagaatggtc

     3481 taaagccagt ttatgatatg gcaaaaacaa tctcctcatt gaacagggtt tgtgctgaga

     3541 tggttgcaaa atatgatctt ctggtgatga caaccggtcg ggcaacagca accgctgcgg

     3601 caactgaggc ttattgggcc gaacatggtc aaccaccacc tggaccatca ctttatgaag

     3661 aaagtgcgat tcggggtaag attgaatcta gagatgagac cgtccctcaa agtgttaggg

     3721 aggcattcaa caatctaaac agtaccactt cactaactga ggaaaatttt gggaaacctg

     3781 acatttcggc aaaggatttg agaaacatta tgtatgatca cttgcctggt tttggaactg

     3841 ctttccacca attagtacaa gtgatttgta aattgggaaa agatagcaac tcattggaca

     3901 tcattcatgc tgagttccag gccagcctgg ctgaaggaga ctctcctcaa tgtgccctaa

     3961 ttcaaattac aaaaagagtt ccaatcttcc aagatgctgc tccacctgtc atccacatcc

     4021 gctctcgagg tgacattccc cgagcttgcc agaaaagctt gcgtccagtc ccaccatcgc

     4081 ccaagattga tcgaggttgg gtatgtgttt ttcagcttca agatggtaaa acacttggac

     4141 tcaaaatttg agccaatctc ccttccctcc gaaagaggcg aataatagca gaggcttcaa

     4201 ctgctgaact atagggtacg ttacattaat gatacacttg tgagtatcag ccctggataa

     4261 tataagtcaa ttaaacgacc aagataaaat tgttcatatc tcgctagcag cttaaaatat

     4321 aaatgtaata ggagctatat ctctgacagt attataatca attgttatta agtaacccaa

     4381 accaaaagtg atgaagatta agaaaaacct acctcggctg agagagtgtt ttttcattaa

     4441 ccttcatctt gtaaacgttg agcaaaattg ttaaaaatat gaggcgggtt atattgccta

     4501 ctgctcctcc tgaatatatg gaggccatat accctgtcag gtcaaattca acaattgcta

     4561 gaggtggcaa cagcaataca ggcttcctga caccggagtc agtcaatggg gacactccat

     4621 cgaatccact caggccaatt gccgatgaca ccatcgacca tgccagccac acaccaggca

     4681 gtgtgtcatc agcattcatc cttgaagcta tggtgaatgt catatcgggc cccaaagtgc

     4741 taatgaagca aattccaatt tggcttcctc taggtgtcgc tgatcaaaag acctacagct

     4801 ttgactcaac tacggccgcc atcatgcttg cttcatacac tatcacccat ttcggcaagg

     4861 caaccaatcc acttgtcaga gtcaatcggc tgggtcctgg aatcccggat catcccctca

     4921 ggctcctgcg aattggaaac caggctttcc tccaggagtt cgttcttccg ccagtccaac

     4981 taccccagta tttcaccttt gatttgacag cactcaaact gatcacccaa ccactgcctg

     5041 ctgcaacatg gaccgatgac actccaacag gatcaaatgg agcgttgcgt ccaggaattt

     5101 catttcatcc aaaacttcgc cccattcttt tacccaacaa aagtgggaag aaggggaaca

     5161 gtgccgatct aacatctccg gagaaaatcc aagcaataat gacttcactc caggacttta

     5221 agatcgttcc aattgatcca accaaaaata tcatgggaat cgaagtgcca gaaactctgg

     5281 tccacaagct gaccggtaag aaggtgactt ctaaaaatgg acaaccaatc atccctgttc

     5341 ttttgccaaa gtacattggg ttggacccgg tggctccagg agacctcacc atggtaatca

     5401 cacaggattg tgacacgtgt cattctcctg caagtcttcc agctgtgatt gagaagtaat

     5461 tgcaataatt gactcagatc cagttttata gaatcttctc agggatagtg ataacatcta

     5521 tttagtaatc cgtccattag aggagacact tttaattgat caatatacta aaggtgcttt

     5581 acaccattgt cttttttctc tcctaaatgt agaacttaac aaaagactca taatatactt

     5641 gtttttaaag gattgattga tgaaagatca taactaataa cattacaaat aatcctacta

     5701 taatcaatac ggtgattcaa atgttaatct ttctcattgc acatactttt tgcccttatc

     5761 ctcaaattgc ctgcatgctt acatctgagg atagccagtg tgacttggat tggaaatgtg

     5821 gagaaaaaat cgggacccat ttctaggttg ttcacaatcc aagtacagac attgcccttc

     5881 taattaagaa aaaatcggcg atgaagatta agccgacagt gagcgtaatc ttcatctctc

     5941 ttagattatt tgttttccag agtaggggtc gtcaggtcct tttcaatcgt gtaaccaaaa

     6001 taaactccac tagaaggata ttgtggggca acaacacaat gggcgttaca ggaatattgc

     6061 agttacctcg tgatcgattc aagaggacat cattctttct ttgggtaatt atccttttcc

     6121 aaagaacatt ttccatccca cttggagtca tccacaatag cacattacag gttagtgatg

     6181 tcgacaaact agtttgtcgt gacaaactgt catccacaaa tcaattgaga tcagttggac

     6241 tgaatctcga agggaatgga gtggcaactg acgtgccatc tgcaactaaa agatggggct

     6301 tcaggtccgg tgtcccacca aaggtggtca attatgaagc tggtgaatgg gctgaaaact

     6361 gctacaatct tgaaatcaaa aaacctgacg ggagtgagtg tctaccagca gcgccagacg

     6421 ggattcgggg cttcccccgg tgccggtatg tgcacaaagt atcaggaacg ggaccgtgtg

     6481 ccggagactt tgccttccat aaagagggtg ctttcttcct gtatgatcga cttgcttcca

     6541 cagttatcta ccgaggaacg actttcgctg aaggtgtcgt tgcatttctg atactgcccc

     6601 aagctaagaa ggacttcttc agctcacacc ccttgagaga gccggtcaat gcaacggagg

     6661 acccgtctag tggctactat tctaccacaa ttagatatca ggctaccggt tttggaacca

     6721 atgagacaga gtacttgttc gaggttgaca atttgaccta cgtccaactt gaatcaagat

     6781 tcacaccaca gtttctgctc cagctgaatg agacaatata tacaagtggg aaaaggagca

     6841 ataccacggg aaaactaatt tggaaggtca accccgaaat tgatacaaca atcggggagt

     6901 gggccttctg ggaaactaaa aaaacctcac tagaaaaatt cgcagtgaag agttgtcttt

     6961 cacagttgta tcaaacggag ccaaaaacat cagtggtcag agtccggcgc gaacttcttc

     7021 cgacccaggg accaacacaa caactgaaga ccacaaaatc atggcttcag aaaattcctc

     7081 tgcaatggtt caagtgcaca gtcaaggaag ggaagctgca gtgtcgcatc taacaaccct

     7141 tgccacaatc tccacgagtc cccaatccct cacaaccaaa ccaggtccgg acaacagcac

     7201 ccataataca cccgtgtata aacttgacat ctctgaggca actcaagttg aacaacatca

     7261 ccgcagaaca gacaacgaca gcacagcctc cgacactccc tctgccacga ccgcagccgg

     7321 acccccaaaa gcagagaaca ccaacacgag caagagcact gacttcctgg accccgccac

     7381 cacaacaagt ccccaaaacc acagcgagac cgctggcaac aacaacactc atcaccaaga

     7441 taccggagaa gagagtgcca gcagcgggaa gctaggctta attaccaata ctattgctgg

     7501 agtcgcagga ctgatcacag gcgggagaag aactcgaaga gaagcaattg tcaatgctca

     7561 acccaaatgc aaccctaatt tacattactg gactactcag gatgaaggtg ctgcaatcgg

     7621 actggcctgg ataccatatt tcgggccagc agccgaggga atttacatag aggggctaat

     7681 gcacaatcaa gatggtttaa tctgtgggtt gagacagctg gccaacgaga cgactcaagc

     7741 tcttcaactg ttcctgagag ccacaactga gctacgcacc ttttcaatcc tcaaccgtaa

     7801 ggcaattgat ttcttgctgc agcgatgggg cggcacatgc cacattctgg gaccggactg

     7861 ctgtatcgaa ccacatgatt ggaccaagaa cataacagac aaaattgatc agattattca

     7921 tgattttgtt gataaaaccc ttccggacca gggggacaat gacaattggt ggacaggatg

     7981 gagacaatgg ataccggcag gtattggagt tacaggcgtt ataattgcag ttatcgcttt

     8041 attctgtata tgcaaatttg tcttttagtt tttcttcaga ttgcttcatg gaaaagctca

     8101 gcctcaaatc aatgaaacca ggatttaatt atatggatta cttgaatcta agattacttg

     8161 acaaatgata atataataca ctggagcttt aaacatagcc aatgtgattc taactccttt

     8221 aaactcacag ttaatcataa acaaggtttg acatcaatct agttatctct ttgagaatga

     8281 taaacttgat gaagattaag aaaaaggtaa tctttcgatt atctttaatc ttcatccttg

     8341 attctacaat catgacagtt gtctttagtg acaagggaaa gaagcctttt tattaagttg

     8401 taataatcag atctgcgaac cggtagagtt tagttgcaac ctaacacaca taaagcattg

     8461 gtcaaaaagt caatagaaat ttaaacagtg agtggagaca acttttaaat ggaagcttca

     8521 tatgagagag gacgcccacg agctgccaga cagcattcaa gggatggaca cgaccaccat

     8581 gttcgagcac gatcatcatc cagagagaat tatcgaggtg agtaccgtca atcaaggagc

     8641 gcctcacaag tgcgcgttcc tactgtattt cataagaaga gagttgaacc attaacagtt

     8701 cctccagcac ctaaagacat atgtccgacc ttgaaaaaag gatttttgtg tgacagtagt

     8761 ttttgcaaaa aagatcacca gttggagagt ttaactgata gggaattact cctactaatc

     8821 gcccgtaaga cttgtggatc agtagaacaa caattaaata taactgcacc caaggactcg

     8881 cgcttagcaa atccaacggc tgatgatttc cagcaagagg aaggtccaaa aattaccttg

     8941 ttgacactga tcaagacggc agaacactgg gcgagacaag acatcagaac catagaggat

     9001 tcaaaattaa gagcattgtt gactctatgt gctgtgatga cgaggaaatt ctcaaaatcc

     9061 cagctgagtc ttttatgtga gacacaccta aggcgcgagg ggcttgggca agatcaggca

     9121 gaacccgttc tcgaagtata tcaacgatta cacagtgata aaggaggcag ttttgaagct

     9181 gcactatggc aacaatggga ccgacaatcc ctaattatgt ttatcactgc attcttgaat

     9241 attgctctcc agttaccgtg tgaaagttct gctgtcgttg tttcagggtt aagaacattg

     9301 gttcctcaat cagataatga ggaagcttca accaacccgg ggacatgctc atggtctgat

     9361 gagggtaccc cttaataagg ctgactaaaa cactatataa ccttctactt gatcacaata

     9421 ctccgtatac ctatcatcat atatttaatc aagacgatat cctttaaaac ttattcagta

     9481 ctataatcac tctcgtttca aattaataag atgtgcatga ttgccctaat atatgaagag

     9541 gtatgataca accctaacag tgatcaaaga aaatcataat ctcgtatcgc tcgtaatata

     9601 acctgccaag catacctctt gcacaaagtg attcttgtac acaaataatg ttttactcta

     9661 caggaggtag caacgatcca tcccatcaaa aaataagtat ttcatgactt actaatgatc

     9721 tcttaaaata ttaagaaaaa ctgacggaac ataaattctt tatgcttcaa gctgtggagg

     9781 aggtgtttgg tattggctat tgttatatta caatcaataa caagcttgta aaaatattgt

     9841 tcttgtttca agaggtagat tgtgaccgga aatgctaaac taatgatgaa gattaatgcg

     9901 gaggtctgat aagaataaac cttattattc agattaggcc ccaagaggca ttcttcatct

     9961 ccttttagca aagtactatt tcagggtagt ccaattagtg gcacgtcttt tagctgtata

    10021 tcagtcgccc ctgagatacg ccacaaaagt gtctctaagc taaattggtc tgtacacatc

    10081 ccatacattg tattaggggc aataatatct aattgaactt agccgtttaa aatttagtgc

    10141 ataaatctgg gctaacacca ccaggtcaac tccattggct gaaaagaagc ttacctacaa

    10201 cgaacatcac tttgagcgcc ctcacaatta aaaaatagga acgtcgttcc aacaatcgag

    10261 cgcaaggttt caaggttgaa ctgagagtgt ctagacaaca aaatattgat actccagaca

    10321 ccaagcaaga cctgagaaaa aaccatggct aaagctacgg gacgatacaa tctaatatcg

    10381 cccaaaaagg acctggagaa aggggttgtc ttaagcgacc tctgtaactt cttagttagc

    10441 caaactattc aggggtggaa ggtttattgg gctggtattg agtttgatgt gactcacaaa

    10501 ggaatggccc tattgcatag actgaaaact aatgactttg cccctgcatg gtcaatgaca

    10561 aggaatctct ttcctcattt atttcaaaat ccgaattcca caattgaatc accgctgtgg

    10621 gcattgagag tcatccttgc agcagggata caggaccagc tgattgacca gtctttgatt

    10681 gaacccttag caggagccct tggtctgatc tctgattggc tgctaacaac caacactaac

    10741 catttcaaca tgcgaacaca acgtgtcaag gaacaattga gcctaaaaat gctgtcgttg

    10801 attcgatcca atattctcaa gtttattaac aaattggatg ctctacatgt cgtgaactac

    10861 aacggattgt tgagcagtat tgaaattgga actcaaaatc atacaatcat cataactcga

    10921 actaacatgg gttttctggt ggagctccaa gaacccgaca aatcggcaat gaaccgcatg

    10981 aagcctgggc cggcgaaatt ttccctcctt catgagtcca cactgaaagc atttacacaa

    11041 ggatcctcga cacgaatgca aagtttgatt cttgaattta atagctctct tgctatctaa

    11101 ctaaggtaga atacttcata ttgagctaac tcatatatgc tgactcaata gttatcttga

    11161 catctctgct ttcataatca gatatataag cataataaat aaatactcat atttcttgat

    11221 aatttgttta accacagata aatcctcact gtaagccagc ttccaagttg acacccttac

    11281 aaaaaccagg actcagaatc cctcaaacaa gagattccaa gacaacatca tagaattgct

    11341 ttattatatg aataagcatt ttatcaccag aaatcctata tactaaatgg ttaattgtaa

    11401 ctgaacccgc aggtcacatg tgttaggttt cacagattct atatattact aactctatac

    11461 tcgtaattaa cattagataa gtagattaag aaaaaagcct gaggaagatt aagaaaaact

    11521 gcttattggg tctttccgtg ttttagatga agcagttgaa attcttcctc ttgatattaa

    11581 atggctacac aacataccca atacccagac gctaggttat catcaccaat tgtattggac

    11641 caatgtgacc tagtcactag agcttgcggg ttatattcat catactccct taatccgcaa

    11701 ctacgcaact gtaaactccc gaaacatatc taccgtttga aatacgatgt aactgttacc

    11761 aagttcttga gtgatgtacc agtggcgaca ttgcccatag atttcatagt cccagttctt

    11821 ctcaaggcac tgtcaggcaa tggattctgt cctgttgagc cgcggtgcca acagttctta

    11881 gatgaaatca ttaagtacac aatgcaagat gctctcttct tgaaatatta tctcaaaaat

    11941 gtgggtgctc aagaagactg tgttgatgaa cactttcaag agaaaatctt atcttcaatt

    12001 cagggcaatg aatttttaca tcaaatgttt ttctggtatg atctggctat tttaactcga

    12061 aggggtagat taaatcgagg aaactctaga tcaacatggt ttgttcatga tgatttaata

    12121 gacatcttag gctatgggga ctatgttttt tggaagatcc caatttcaat gttaccactg

    12181 aacacacaag gaatccccca tgctgctatg gactggtatc aggcatcagt attcaaagaa

    12241 gcggttcaag ggcatacaca cattgtttct gtttctactg ccgacgtctt gataatgtgc

    12301 aaagatttaa ttacatgtcg attcaacaca actctaatct caaaaatagc agagattgag

    12361 gatccagttt gttctgatta tcccaatttt aagattgtgt ctatgcttta ccagagcgga

    12421 gattacttac tctccatatt agggtctgat gggtataaaa ttattaagtt cctcgaacca

    12481 ttgtgcttgg ccaaaattca attatgctca aagtacactg agaggaaggg ccgattctta

    12541 acacaaatgc atttagctgt aaatcacacc ctagaagaaa ttacagaaat gcgtgcacta

    12601 aagccttcac aggctcaaaa gatccgtgaa ttccatagaa cattgataag gctggagatg

    12661 acgccacaac aactttgtga gctattttcc attcaaaaac actgggggca tcctgtgcta

    12721 catagtgaaa cagcaatcca aaaagttaaa aaacatgcta cggtgctaaa agcattacgc

    12781 cctatagtga ttttcgagac atactgtgtt tttaaatata gtattgccaa acattatttt

    12841 gatagtcaag gatcttggta cagtgttact tcagatagga atctaacacc gggtcttaat

    12901 tcttatatca aaagaaatca attccctccg ttgccaatga ttaaagaact actatgggaa

    12961 ttttaccacc ttgaccaccc tccacttttc tcaaccaaaa ttattagtga cttaagtatt

    13021 tttataaaag acagagctac cgcagtagaa aggacatgct gggatgcagt attcgagcct

    13081 aatgttctag gatataatcc acctcacaaa tttagtacta aacgtgtacc ggaacaattt

    13141 ttagagcaag aaaacttttc tattgagaat gttctttcct acgcacaaaa actcgagtat

    13201 ctactaccac aatatcggaa cttttctttc tcattgaaag agaaagagtt gaatgtaggt

    13261 agaaccttcg gaaaattgcc ttatccgact cgcaatgttc aaacactttg tgaagctctg

    13321 ttagctgatg gtcttgctaa agcatttcct agcaatatga tggtagttac ggaacgtgag

    13381 caaaaagaaa gcttattgca tcaagcatca tggcaccaca caagtgatga ttttggtgaa

    13441 catgccacag ttagagggag tagctttgta actgatttag agaaatacaa tcttgcattt

    13501 agatatgagt ttacagcacc ttttatagaa tattgcaacc gttgctatgg tgttaagaat

    13561 gtttttaatt ggatgcatta tacaatccca cagtgttata tgcatgtcag tgattattat

    13621 aatccaccac ataacctcac actggagaat cgagacaacc cccccgaagg gcctagttca

    13681 tacaggggtc atatgggagg gattgaagga ctgcaacaaa aactctggac aagtatttca

    13741 tgtgctcaaa tttctttagt tgaaattaag actggtttta agttacgctc agctgtgatg

    13801 ggtgacaatc agtgcattac tgttttatca gtcttcccct tagagactga cgcagacgag

    13861 caggaacaga gcgccgaaga caatgcagcg agggtggccg ccagcctagc aaaagttaca

    13921 agtgcctgtg gaatcttttt aaaacctgat gaaacatttg tacattcagg ttttatctat

    13981 tttggaaaaa aacaatattt gaatggggtc caattgcctc agtcccttaa aacggctaca

    14041 agaatggcac cattgtctga tgcaattttt gatgatcttc aagggaccct ggctagtata

    14101 ggcactgctt ttgagcgatc catctctgag acacgacata tctttccttg caggataacc

    14161 gcagctttcc atacgttttt ttcggtgaga atcttgcaat atcatcatct cgggttcaat

    14221 aaaggttttg accttggaca gttaacactc ggcaaacctc tggatttcgg aacaatatca

    14281 ttggcactag cggtaccgca ggtgcttgga gggttatcct tcttgaatcc tgagaaatgt

    14341 ttctaccgga atctaggaga tccagttacc tcaggcttat tccagttaaa aacttatctc

    14401 cgaatgattg agatggatga tttattctta cctttaattg cgaagaaccc tgggaactgc

    14461 actgccattg actttgtgct aaatcctagc ggattaaatg tccctgggtc gcaagactta

    14521 acttcatttc tgcgccagat tgtacgcagg accatcaccc taagtgcgaa aaacaaactt

    14581 attaatacct tatttcatgc gtcagctgac ttcgaagacg aaatggtttg taaatggcta

    14641 ttatcatcaa ctcctgttat gagtcgtttt gcggccgata tcttttcacg cacgccgagc

    14701 gggaagcgat tgcaaattct aggatacctg gaaggaacac gcacattatt agcctctaag

    14761 atcatcaaca ataatacaga gacaccggtt ttggacagac tgaggaaaat aacattgcaa

    14821 aggtggagcc tatggtttag ttatcttgat cattgtgata atatcctggc ggaggcttta

    14881 acccaaataa cttgcacagt tgatttagca cagattctga gggaatattc atgggctcat

    14941 attttagagg gaagacctct tattggagcc acactcccat gtatgattga gcaattcaaa

    15001 gtgttttggc tgaaacccta cgaacaatgt ccgcagtgtt caaatgcaaa gcaaccaggt

    15061 gggaaaccat tcgtgtcagt ggcagtcaag aaacatattg ttagtgcatg gccgaacgca

    15121 tcccgaataa gctggactat cggggatgga atcccataca ttggatcaag gacagaagat

    15181 aagataggac aacctgctat taaaccaaaa tgtccttccg cagccttaag agaggccatt

    15241 gaattggcgt cccgtttaac atgggtaact caaggcagtt cgaacagtga cttgctaata

    15301 aaaccatttt tggaagcacg agtaaattta agtgttcaag aaatacttca aatgacccct

    15361 tcacattact caggaaatat tgttcacagg tacaacgatc aatacagtcc tcattctttc

    15421 atggccaatc gtatgagtaa ttcagcaacg cgattgattg tttctacaaa cactttaggt

    15481 gagttttcag gaggtggcca gtctgcacgc gacagcaata ttattttcca gaatgttata

    15541 aattatgcag ttgcactgtt cgatattaaa tttagaaaca ctgaggctac agatatccaa

    15601 tataatcgtg ctcaccttca tctaactaag tgttgcaccc gggaagtacc agctcagtat

    15661 ttaacataca catctacatt ggatttagat ttaacaagat accgagaaaa cgaattgatt

    15721 tatgacagta atcctctaaa aggaggactc aattgcaata tctcattcga taatccattt

    15781 ttccaaggta aacggctgaa cattatagaa gatgatctta ttcgactgcc tcacttatct

    15841 ggatgggagc tagccaagac catcatgcaa tcaattattt cagatagcaa caattcatct

    15901 acagacccaa ttagcagtgg agaaacaaga tcattcacta cccatttctt aacttatccc

    15961 aagataggac ttctgtacag ttttggggcc tttgtaagtt attatcttgg caatacaatt

    16021 cttcggacta agaaattaac acttgacaat tttttatatt acttaactac tcaaattcat

    16081 aatctaccac atcgctcatt gcgaatactt aagccaacat tcaaacatgc aagcgttatg

    16141 tcacggttaa tgagtattga tcctcatttt tctatttaca taggcggtgc tgcaggtgac

    16201 agaggactct cagatgcggc caggttattt ttgagaacgt ccatttcatc ttttcttaca

    16261 tttgtaaaag aatggataat taatcgcgga acaattgtcc ctttatggat agtatatccg

    16321 ctagagggtc aaaacccaac acctgtgaat aattttctct atcagatcgt agaactgctg

    16381 gtgcatgatt catcaagaca acaggctttt aaaactacca taagtgatca tgtacatcct

    16441 cacgacaatc ttgtttacac atgtaagagt acagccagca atttcttcca tgcatcattg

    16501 gcgtactgga ggagcagaca cagaaacagc aaccgaaaat acttggcaag agactcttca

    16561 actggatcaa gcacaaacaa cagtgatggt catattgaga gaagtcaaga acaaaccacc

    16621 agagatccac atgatggcac tgaacggaat ctagtcctac aaatgagcca tgaaataaaa

    16681 agaacgacaa ttccacaaga aaacacgcac cagggtccgt cgttccagtc ctttctaagt

    16741 gactctgctt gtggtacagc aaatccaaaa ctaaatttcg atcgatcgag acacaatgtg

    16801 aaatttcagg atcataactc ggcatccaag agggaaggtc atcaaataat ctcacaccgt

    16861 ctagtcctac ctttctttac attatctcaa gggacacgcc aattaacgtc atccaatgag

    16921 tcacaaaccc aagacgagat atcaaagtac ttacggcaat tgagatccgt cattgatacc

    16981 acagtttatt gtagatttac cggtatagtc tcgtccatgc attacaaact tgatgaggtc

    17041 ctttgggaaa tagagagttt caagtcggct gtgacgctag cagagggaga aggtgctggt

    17101 gccttactat tgattcagaa ataccaagtt aagaccttat ttttcaacac gctagctact

    17161 gagtccagta tagagtcaga aatagtatca ggaatgacta ctcctaggat gcttctacct

    17221 gttatgtcaa aattccataa tgaccaaatt gagattattc ttaacaactc agcaagccaa

    17281 ataacagaca taacaaatcc tacttggttt aaagaccaaa gagcaaggct acctaagcaa

    17341 gtcgaggtta taaccatgga tgcagagaca acagagaata taaacagatc gaaattgtac

    17401 gaagctgtat ataaattgat cttacaccat attgatccta gcgtattgaa agcagtggtc

    17461 cttaaagtct ttctaagtga tactgagggt atgttatggc taaatgataa tttagccccg

    17521 ttttttgcca ctggttattt aattaagcca ataacgtcaa gtgctagatc tagtgagtgg

    17581 tatctttgtc tgacgaactt cttatcaact acacgtaaga tgccacacca aaaccatctc

    17641 agttgtaaac aggtaatact tacggcattg caactgcaaa ttcaacgaag cccatactgg

    17701 ctaagtcatt taactcagta tgctgactgt gagttacatt taagttatat ccgccttggt

    17761 tttccatcat tagagaaagt actataccac aggtataacc tcgtcgattc aaaaagaggt

    17821 ccactagtct ctatcactca gcacttagca catcttagag cagagattcg agaattaact

    17881 aatgattata atcaacagcg acaaagtcgg actcaaacat atcactttat tcgtactgca

    17941 aaaggacgaa tcacaaaact agtcaatgat tatttaaaat tctttcttat tgtgcaagca

    18001 ttaaaacata atgggacatg gcaagctgag tttaagaaat taccagagtt gattagtgtg

    18061 tgcaataggt tctaccatat tagagattgc aattgtgaag aacgtttctt agttcaaacc

    18121 ttatatttac atagaatgca ggattctgaa gttaagctta tcgaaaggct gacagggctt

    18181 ctgagtttat ttccggatgg tctctacagg tttgattgaa ttaccgtgca tagtatcctg

    18241 atacttgcaa aggttggtta ttaacataca gattataaaa aactcataaa ttgctctcat

    18301 acatcatatt gatctaatct caataaacaa ctatttaaat aacgaaagga gtccctatat

    18361 tatatactat atttagcctc tctccctgcg tgataatcaa aaaattcaca atgcagcatg

    18421 tgtgacatat tactgccgca atgaatttaa cgcaacataa taaactctgc actctttata

    18481 attaagcttt aacgaaaggt ctgggctcat attgttattg atataataat gttgtatcaa

    18541 tatcctgtca gatggaatag tgttttggtt gataacacaa cttcttaaaa caaaattgat

    18601 ctttaagatt aagtttttta taattatcat tactttaatt tgtcgtttta aaaacggtga

    18661 tagccttaat ctttgtgtaa aataagagat taggtgtaat aaccttaaca tttttgtcta

    18721 gtaagctact atttcataca gaatgataaa attaaaagaa aaggcaggac tgtaaaatca

    18781 gaaatacctt ctttacaata tagcagacta gataataatc ttcgtgttaa tgataattaa

    18841 gacattgacc acgctcatca gaaggctcgc cagaataaac gttgcaaaaa ggattcctgg

    18901 aaaaatggtc gcacacaaaa atttaaaaat aaatctattt cttctttttt gtgtgtcca

 

//Ebola Genome

Zaire ebolavirus isolate Ebola virus H.sapiens-tc/COD/1976/Yambuku-Mayinga, complete genome. NCBI Reference Sequence: NC_002549.1. 

http://www.ncbi.nlm.nih.gov/nuccore/10313991?report=graph

LOCUS       NC_002549              18959 bp    cRNA    linear   VRL 27-AUG-2014

DEFINITION  Zaire ebolavirus isolate Ebola virus

            H.sapiens-tc/COD/1976/Yambuku-Mayinga, complete genome.

ACCESSION   NC_002549

VERSION     NC_002549.1  GI:10313991

DBLINK      BioProject: PRJNA14703

KEYWORDS    RefSeq.

SOURCE      Zaire ebolavirus (ZEBOV)

  ORGANISM  Zaire ebolavirus

            Viruses; ssRNA negative-strand viruses; Mononegavirales;

            Filoviridae; Ebolavirus.

REFERENCE   1  (bases 1 to 18959)

  AUTHORS   Volchkov,V.E., Volchkova,V.A., Chepurnov,A.A., Blinov,V.M.,

            Dolnik,O., Netesov,S.V. and Feldmann,H.

  TITLE     Characterization of the L gene and 5' trailer region of Ebola virus

  JOURNAL   J. Gen. Virol. 80 (Pt 2), 355-362 (1999)

   PUBMED   10073695

REFERENCE   2  (bases 1 to 18959)

  AUTHORS   Volchkov,V.E., Volchkova,V.A., Slenczka,W., Klenk,H.D. and

            Feldmann,H.

  TITLE     Release of viral glycoproteins during Ebola virus infection

  JOURNAL   Virology 245 (1), 110-119 (1998)

   PUBMED   9614872

REFERENCE   3  (bases 1 to 18959)

  AUTHORS   Volchkov,V.E., Feldmann,H., Volchkova,V.A. and Klenk,H.D.

  TITLE     Processing of the Ebola virus glycoprotein by the proprotein

            convertase furin

  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 95 (10), 5762-5767 (1998)

   PUBMED   9576958

REFERENCE   4  (bases 1 to 18959)

  AUTHORS   Volchkov,V.E., Becker,S., Volchkova,V.A., Ternovoj,V.A.,

            Kotov,A.N., Netesov,S.V. and Klenk,H.D.

  TITLE     GP mRNA of Ebola virus is edited by the Ebola virus polymerase and

            by T7 and vaccinia virus polymerases

  JOURNAL   Virology 214 (2), 421-430 (1995)

   PUBMED   8553543

REFERENCE   5  (bases 1 to 18959)

  AUTHORS   Bukreyev,A.A., Volchkov,V.E., Blinov,V.M. and Netesov,S.V.

  TITLE     The VP35 and VP40 proteins of filoviruses. Homology between Marburg

            and Ebola viruses

  JOURNAL   FEBS Lett. 322 (1), 41-46 (1993)

   PUBMED   8482365

REFERENCE   6  (bases 1 to 18959)

  CONSRTM   NCBI Genome Project

  TITLE     Direct Submission

  JOURNAL   Submitted (27-SEP-2000) National Center for Biotechnology

            Information, NIH, Bethesda, MD 20894, USA

REFERENCE   7  (bases 1 to 18959)

  AUTHORS   Volchkov,V.E.

  TITLE     Direct Submission

  JOURNAL   Submitted (02-JUN-2000) Institute of Virology, Philipps-University

            Marburg, Robert-Koch-Str. 17, Marburg 35037, Germany

  REMARK    Sequence update by submitter

REFERENCE   8  (bases 1 to 18959)

  AUTHORS   Volchkov,V.E.

  TITLE     Direct Submission

  JOURNAL   Submitted (20-AUG-1998) Institute of Virology, Philipps-University

            Marburg, Robert-Koch-Str. 17, Marburg 35037, Germany

COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final

            NCBI review. The reference sequence is identical to AF086833.

            COMPLETENESS: full length.

FEATURES             Location/Qualifiers

     source          1..18959

                     /organism="Zaire ebolavirus"

                     /mol_type="viral cRNA"

                     /isolate="Ebola virus

                     H.sapiens-tc/COD/1976/Yambuku-Mayinga"

                     /db_xref="taxon:186538"

     5'UTR           1..55

                     /note="putative leader region"

                     /citation=[1]

                     /function="regulation or initiation of RNA replication"

     gene            56..3026

                     /gene="NP"

                     /locus_tag="ZEBOVgp1"

                     /db_xref="GeneID:911830"

     mRNA            56..3026

                     /gene="NP"

                     /locus_tag="ZEBOVgp1"

                     /product="nucleoprotein"

                     /db_xref="GeneID:911830"

     misc_signal     56..67

                     /gene="NP"

                     /locus_tag="ZEBOVgp1"

                     /note="putative; transcription start signal"

                     /citation=[1]

     CDS             470..2689

                     /gene="NP"

                     /locus_tag="ZEBOVgp1"

                     /function="encapsidation of genomic RNA"

                     /codon_start=1

                     /product="nucleoprotein"

                     /protein_id="NP_066243.1"

                     /db_xref="GI:10314000"

                     /db_xref="GeneID:911830"

                     /translation="MDSRPQKIWMAPSLTESDMDYHKILTAGLSVQQGIVRQRVIPVY

                     QVNNLEEICQLIIQAFEAGVDFQESADSFLLMLCLHHAYQGDYKLFLESGAVKYLEGH

                     GFRFEVKKRDGVKRLEELLPAVSSGKNIKRTLAAMPEEETTEANAGQFLSFASLFLPK

                     LVVGEKACLEKVQRQIQVHAEQGLIQYPTAWQSVGHMMVIFRLMRTNFLIKFLLIHQG

                     MHMVAGHDANDAVISNSVAQARFSGLLIVKTVLDHILQKTERGVRLHPLARTAKVKNE

                     VNSFKAALSSLAKHGEYAPFARLLNLSGVNNLEHGLFPQLSAIALGVATAHGSTLAGV

                     NVGEQYQQLREAATEAEKQLQQYAESRELDHLGLDDQEKKILMNFHQKKNEISFQQTN

                     AMVTLRKERLAKLTEAITAASLPKTSGHYDDDDDIPFPGPINDDDNPGHQDDDPTDSQ

                     DTTIPDVVVDPDDGSYGEYQSYSENGMNAPDDLVLFDLDEDDEDTKPVPNRSTKGGQQ

                     KNSQKGQHIEGRQTQSRPIQNVPGPHRTIHHASAPLTDNDRRNEPSGSTSPRMLTPIN

                     EEADPLDDADDETSSLPPLESDDEEQDRDGTSNRTPTVAPPAPVYRDHSEKKELPQDE

                     QQDQDHTQEARNQDSDNTQSEHSFEEMYRHILRSQGPFDAVLYYHMMKDEPVVFSTSD

                     GKEYTYPDSLEEEYPPWLTEKEAMNEENRFVTLDGQQFYWPVMNHKNKFMAILQHHQ"

     misc_feature    524..2671

                     /gene="NP"

                     /locus_tag="ZEBOVgp1"

                     /note="Ebola nucleoprotein; Region: Ebola_NP; pfam05505"

                     /db_xref="CDD:147601"

     polyA_signal    3015..3026

                     /gene="NP"

                     /locus_tag="ZEBOVgp1"

     misc_feature    3027..3031

                     /note="intergenic region"

     gene            3032..4407

                     /gene="VP35"

                     /locus_tag="ZEBOVgp2"

                     /db_xref="GeneID:911827"

     mRNA            3032..4407

                     /gene="VP35"

                     /locus_tag="ZEBOVgp2"

                     /product="VP35"

                     /citation=[5]

                     /db_xref="GeneID:911827"

     misc_signal     3032..3043

                     /gene="VP35"

                     /locus_tag="ZEBOVgp2"

                     /note="putative; transcription start signal"

                     /citation=[5]

     CDS             3129..4151

                     /gene="VP35"

                     /locus_tag="ZEBOVgp2"

                     /function="polymerase complex protein"

                     /citation=[5]

                     /codon_start=1

                     /product="polymerase complex protein"

                     /protein_id="NP_066244.1"

                     /db_xref="GI:10313992"

                     /db_xref="GeneID:911827"

                     /translation="MTTRTKGRGHTAATTQNDRMPGPELSGWISEQLMTGRIPVSDIF

                     CDIENNPGLCYASQMQQTKPNPKTRNSQTQTDPICNHSFEEVVQTLASLATVVQQQTI

                     ASESLEQRITSLENGLKPVYDMAKTISSLNRVCAEMVAKYDLLVMTTGRATATAAATE

                     AYWAEHGQPPPGPSLYEESAIRGKIESRDETVPQSVREAFNNLNSTTSLTEENFGKPD

                     ISAKDLRNIMYDHLPGFGTAFHQLVQVICKLGKDSNSLDIIHAEFQASLAEGDSPQCA

                     LIQITKRVPIFQDAAPPVIHIRSRGDIPRACQKSLRPVPPSPKIDRGWVCVFQLQDGK

                     TLGLKI"

     misc_feature    3186..4148

                     /gene="VP35"

                     /locus_tag="ZEBOVgp2"

                     /note="Filoviridae VP35; Region: Filo_VP35; pfam02097"

                     /db_xref="CDD:145320"

     gene            4390..5894

                     /gene="VP40"

                     /locus_tag="ZEBOVgp3"

                     /db_xref="GeneID:911825"

     mRNA            4390..5894

                     /gene="VP40"

                     /locus_tag="ZEBOVgp3"

                     /product="VP40"

                     /citation=[5]

                     /db_xref="GeneID:911825"

     misc_signal     4390..4401

                     /gene="VP40"

                     /locus_tag="ZEBOVgp3"

                     /note="transcription start signal"

                     /citation=[5]

     polyA_signal    4397..4407

                     /gene="VP35"

                     /locus_tag="ZEBOVgp2"

                     /citation=[5]

     CDS             4479..5459

                     /gene="VP40"

                     /locus_tag="ZEBOVgp3"

                     /citation=[5]

                     /codon_start=1

                     /product="matrix protein"

                     /protein_id="NP_066245.1"

                     /db_xref="GI:10313993"

                     /db_xref="GeneID:911825"

                     /translation="MRRVILPTAPPEYMEAIYPVRSNSTIARGGNSNTGFLTPESVNG

                     DTPSNPLRPIADDTIDHASHTPGSVSSAFILEAMVNVISGPKVLMKQIPIWLPLGVAD

                     QKTYSFDSTTAAIMLASYTITHFGKATNPLVRVNRLGPGIPDHPLRLLRIGNQAFLQE

                     FVLPPVQLPQYFTFDLTALKLITQPLPAATWTDDTPTGSNGALRPGISFHPKLRPILL

                     PNKSGKKGNSADLTSPEKIQAIMTSLQDFKIVPIDPTKNIMGIEVPETLVHKLTGKKV

                     TSKNGQPIIPVLLPKYIGLDPVAPGDLTMVITQDCDTCHSPASLPAVIEK"

     misc_feature    4479..5363

                     /gene="VP40"

                     /locus_tag="ZEBOVgp3"

                     /note="Matrix protein VP40; Region: VP40; pfam07447"

                     /db_xref="CDD:116068"

     polyA_signal    5883..5894

                     /gene="VP40"

                     /locus_tag="ZEBOVgp3"

                     /citation=[5]

     misc_feature    5895..5899

                     /note="intergenic region"

     gene            5900..8305

                     /gene="GP"

                     /locus_tag="ZEBOVgp4"

                     /db_xref="GeneID:911829"

     mRNA            5900..8305

                     /gene="GP"

                     /locus_tag="ZEBOVgp4"

                     /product="sGP"

                     /note="unedited mRNA"

                     /citation=[4]

                     /db_xref="GeneID:911829"

     misc_signal     5900..5911

                     /gene="GP"

                     /locus_tag="ZEBOVgp4"

                     /note="putative; transcription start signal"

                     /citation=[4]

     CDS             join(6039..6923,6923..8068)

                     /gene="GP"

                     /locus_tag="ZEBOVgp4"

                     /function="receptor binding and fusion"

                     /artificial_location="low-quality sequence region"

                     /note="virion spike glycoprotein precursor; an addition A

                     residue is inserted during transcription; encodes two

                     disulfide linked subunits GP1 and GP2"

                     /citation=[2]

                     /citation=[3]

                     /citation=[4]

                     /codon_start=1

                     /product="spike glycoprotein"

                     /protein_id="NP_066246.1"

                     /db_xref="GI:10313995"

                     /db_xref="GeneID:911829"

                     /translation="MGVTGILQLPRDRFKRTSFFLWVIILFQRTFSIPLGVIHNSTLQ

                     VSDVDKLVCRDKLSSTNQLRSVGLNLEGNGVATDVPSATKRWGFRSGVPPKVVNYEAG

                     EWAENCYNLEIKKPDGSECLPAAPDGIRGFPRCRYVHKVSGTGPCAGDFAFHKEGAFF

                     LYDRLASTVIYRGTTFAEGVVAFLILPQAKKDFFSSHPLREPVNATEDPSSGYYSTTI

                     RYQATGFGTNETEYLFEVDNLTYVQLESRFTPQFLLQLNETIYTSGKRSNTTGKLIWK

                     VNPEIDTTIGEWAFWETKKNLTRKIRSEELSFTVVSNGAKNISGQSPARTSSDPGTNT

                     TTEDHKIMASENSSAMVQVHSQGREAAVSHLTTLATISTSPQSLTTKPGPDNSTHNTP

                     VYKLDISEATQVEQHHRRTDNDSTASDTPSATTAAGPPKAENTNTSKSTDFLDPATTT

                     SPQNHSETAGNNNTHHQDTGEESASSGKLGLITNTIAGVAGLITGGRRTRREAIVNAQ

                     PKCNPNLHYWTTQDEGAAIGLAWIPYFGPAAEGIYIEGLMHNQDGLICGLRQLANETT

                     QALQLFLRATTELRTFSILNRKAIDFLLQRWGGTCHILGPDCCIEPHDWTKNITDKID

                     QIIHDFVDKTLPDQGDNDNWWTGWRQWIPAGIGVTGVIIAVIALFCICKFVF"

     misc_feature    7529..7540

                     /gene="GP"

                     /locus_tag="ZEBOVgp4"

                     /note="encodes the glycoprotein cleavage site, precursor

                     GP is cleaved by subtilisin-like cellular protease furin

                     into subunits GP1 and GP2 that are linked by a disulfide

                     bond"

                     /citation=[3]

     misc_feature    7793..7870

                     /gene="GP"

                     /locus_tag="ZEBOVgp4"

                     /note="immunosuppressive motif; other site"

     misc_feature    7988..8053

                     /gene="GP"

                     /locus_tag="ZEBOVgp4"

                     /note="transmembrane anchor; transmembrane region"

     misc_feature    7706..7924

                     /gene="GP"

                     /locus_tag="ZEBOVgp4"

                     /note="heptad repeat 1-heptad repeat 2 region of the

                     transmembrane subunit of Filoviridae viruses, Ebola virus

                     and Marburg virus, and related domains; Region:

                     Ebola-like_HR1-HR2; cd09850"

                     /db_xref="CDD:197367"

     misc_feature    join(6081..6923,6923..7153)

                     /gene="GP"

                     /locus_tag="ZEBOVgp4"

                     /note="Filovirus glycoprotein; Region: Filo_glycop;

                     pfam01611"

                     /db_xref="CDD:110602"

     misc_feature    7706..7732

                     /gene="GP"

                     /locus_tag="ZEBOVgp4"

                     /note="HR1A; other site"

                     /db_xref="CDD:197367"

     misc_feature    7733..7762

                     /gene="GP"

                     /locus_tag="ZEBOVgp4"

                     /note="HR1B; other site"

                     /db_xref="CDD:197367"

     misc_feature    7763..7783

                     /gene="GP"

                     /locus_tag="ZEBOVgp4"

                     /note="HR1C; other site"

                     /db_xref="CDD:197367"

     misc_feature    7784..7831

                     /gene="GP"

                     /locus_tag="ZEBOVgp4"

                     /note="HR1D; other site"

                     /db_xref="CDD:197367"

     misc_feature    7787..7837

                     /gene="GP"

                     /locus_tag="ZEBOVgp4"

                     /note="immunosuppressive region; other site"

                     /db_xref="CDD:197367"

     misc_feature    order(7838..7858,7859..7861)

                     /gene="GP"

                     /locus_tag="ZEBOVgp4"

                     /note="CX(6,7)C motif; other site"

                     /db_xref="CDD:197367"

     misc_feature    7886..7924

                     /gene="GP"

                     /locus_tag="ZEBOVgp4"

                     /note="HR2; other site"

                     /db_xref="CDD:197367"

     misc_feature    order(7784..7786,7793..7795)

                     /gene="GP"

                     /locus_tag="ZEBOVgp4"

                     /note="Cl binding site [ion binding]; other site"

                     /db_xref="CDD:197367"

     misc_feature    order(7706..7714,7718..7723,7727..7732,7736..7744,

                     7748..7756,7760..7765,7769..7777,7781..7807,7811..7819,

                     7823..7828,7844..7849,7856..7858,7865..7876,7880..7882,

                     7889..7894,7901..7903,7910..7915,7922..7924)

                     /gene="GP"

                     /locus_tag="ZEBOVgp4"

                     /note="homotrimer interface [polypeptide binding]; other

                     site"

                     /db_xref="CDD:197367"

     misc_feature    order(7706..7714,7718..7726,7730..7735,7739..7747,

                     7754..7768,7772..7783,7787..7792,7796..7804,7808..7813,

                     7817..7819)

                     /gene="GP"

                     /locus_tag="ZEBOVgp4"

                     /note="HR1-GP1 interface [polypeptide binding]; other

                     site"

                     /db_xref="CDD:197367"

     CDS             6039..7133

                     /gene="GP"

                     /locus_tag="ZEBOVgp4"

                     /note="sGP, small non-structural, secreted glycoprotein;

                     sGP secreted as a anti-parallel oriented homodimer"

                     /citation=[4]

                     /codon_start=1

                     /product="small secreted glycoprotein"

                     /protein_id="NP_066247.1"

                     /db_xref="GI:10313994"

                     /db_xref="GeneID:911829"

                     /translation="MGVTGILQLPRDRFKRTSFFLWVIILFQRTFSIPLGVIHNSTLQ

                     VSDVDKLVCRDKLSSTNQLRSVGLNLEGNGVATDVPSATKRWGFRSGVPPKVVNYEAG

                     EWAENCYNLEIKKPDGSECLPAAPDGIRGFPRCRYVHKVSGTGPCAGDFAFHKEGAFF

                     LYDRLASTVIYRGTTFAEGVVAFLILPQAKKDFFSSHPLREPVNATEDPSSGYYSTTI

                     RYQATGFGTNETEYLFEVDNLTYVQLESRFTPQFLLQLNETIYTSGKRSNTTGKLIWK

                     VNPEIDTTIGEWAFWETKKTSLEKFAVKSCLSQLYQTEPKTSVVRVRRELLPTQGPTQ

                     QLKTTKSWLQKIPLQWFKCTVKEGKLQCRI"

     misc_feature    6081..7130

                     /gene="GP"

                     /locus_tag="ZEBOVgp4"

                     /note="Filovirus glycoprotein; Region: Filo_glycop;

                     pfam01611"

                     /db_xref="CDD:110602"

     CDS             join(6039..6922,6924..6933)

                     /gene="GP"

                     /locus_tag="ZEBOVgp4"

                     /artificial_location="low-quality sequence region"

                     /note="ssGP; second non-structural secreted glycoprotein;

                     secreted in a monomeric form; one A residue is deleted or

                     two additional A residues are inserted at the editing site

                     during transcription of the GP gene"

                     /citation=[4]

                     /codon_start=1

                     /product="second secreted glycoprotein"

                     /protein_id="NP_066248.1"

                     /db_xref="GI:10313996"

                     /db_xref="GeneID:911829"

                     /translation="MGVTGILQLPRDRFKRTSFFLWVIILFQRTFSIPLGVIHNSTLQ

                     VSDVDKLVCRDKLSSTNQLRSVGLNLEGNGVATDVPSATKRWGFRSGVPPKVVNYEAG

                     EWAENCYNLEIKKPDGSECLPAAPDGIRGFPRCRYVHKVSGTGPCAGDFAFHKEGAFF

                     LYDRLASTVIYRGTTFAEGVVAFLILPQAKKDFFSSHPLREPVNATEDPSSGYYSTTI

                     RYQATGFGTNETEYLFEVDNLTYVQLESRFTPQFLLQLNETIYTSGKRSNTTGKLIWK

                     VNPEIDTTIGEWAFWETKKPH"

     misc_feature    join(6081..6922,6924..>6924)

                     /gene="GP"

                     /locus_tag="ZEBOVgp4"

                     /note="Filovirus glycoprotein; Region: Filo_glycop;

                     pfam01611"

                     /db_xref="CDD:110602"

     misc_signal     6918..6924

                     /gene="GP"

                     /locus_tag="ZEBOVgp4"

                     /note="additional A residues are inserted or deleted

                     during transcription of the GP gene by the viral

                     polymerase"

                     /citation=[4]

                     /function="RNA editing"

     gene            8288..9740

                     /gene="VP30"

                     /locus_tag="ZEBOVgp5"

                     /db_xref="GeneID:911826"

     mRNA            8288..9740

                     /gene="VP30"

                     /locus_tag="ZEBOVgp5"

                     /product="VP30"

                     /db_xref="GeneID:911826"

     misc_signal     8288..8299

                     /gene="VP30"

                     /locus_tag="ZEBOVgp5"

                     /note="putative; transcription start signal"

     polyA_signal    8295..8305

                     /gene="GP"

                     /locus_tag="ZEBOVgp4"

                     /citation=[4]

     CDS             8509..9375

                     /gene="VP30"

                     /locus_tag="ZEBOVgp5"

                     /note="polymerase complex protein"

                     /codon_start=1

                     /product="minor nucleoprotein"

                     /protein_id="NP_066249.1"

                     /db_xref="GI:10313997"

                     /db_xref="GeneID:911826"

                     /translation="MEASYERGRPRAARQHSRDGHDHHVRARSSSRENYRGEYRQSRS

                     ASQVRVPTVFHKKRVEPLTVPPAPKDICPTLKKGFLCDSSFCKKDHQLESLTDRELLL

                     LIARKTCGSVEQQLNITAPKDSRLANPTADDFQQEEGPKITLLTLIKTAEHWARQDIR

                     TIEDSKLRALLTLCAVMTRKFSKSQLSLLCETHLRREGLGQDQAEPVLEVYQRLHSDK

                     GGSFEAALWQQWDRQSLIMFITAFLNIALQLPCESSAVVVSGLRTLVPQSDNEEASTN

                     PGTCSWSDEGTP"

     misc_feature    8932..9321

                     /gene="VP30"

                     /locus_tag="ZEBOVgp5"

                     /note="Ebola virus-specific transcription factor VP30;

                     Region: Transcript_VP30; pfam11507"

                     /db_xref="CDD:151944"

     polyA_signal    9730..9740

                     /gene="VP30"

                     /locus_tag="ZEBOVgp5"

                     /note="putative"

     misc_feature    9741..9884

                     /note="intergenic region"

     gene            9885..11518

                     /gene="VP24"

                     /locus_tag="ZEBOVgp6"

                     /note="putative"

                     /db_xref="GeneID:911828"

     mRNA            9885..11496

                     /gene="VP24"

                     /locus_tag="ZEBOVgp6"

                     /product="VP24"

                     /db_xref="GeneID:911828"

     misc_signal     9885..9896

                     /gene="VP24"

                     /locus_tag="ZEBOVgp6"

                     /note="transcription start signal"

     CDS             10345..11100

                     /gene="VP24"

                     /locus_tag="ZEBOVgp6"

                     /codon_start=1

                     /product="membrane-associated protein"

                     /protein_id="NP_066250.1"

                     /db_xref="GI:10313998"

                     /db_xref="GeneID:911828"

                     /translation="MAKATGRYNLISPKKDLEKGVVLSDLCNFLVSQTIQGWKVYWAG

                     IEFDVTHKGMALLHRLKTNDFAPAWSMTRNLFPHLFQNPNSTIESPLWALRVILAAGI

                     QDQLIDQSLIEPLAGALGLISDWLLTTNTNHFNMRTQRVKEQLSLKMLSLIRSNILKF

                     INKLDALHVVNYNGLLSSIEIGTQNHTIIITRTNMGFLVELQEPDKSAMNRMKPGPAK

                     FSLLHESTLKAFTQGSSTRMQSLILEFNSSLAI"

     misc_feature    10369..11040

                     /gene="VP24"

                     /locus_tag="ZEBOVgp6"

                     /note="Filovirus membrane-associated protein VP24; Region:

                     Filo_VP24; pfam06389"

                     /db_xref="CDD:253701"

     polyA_signal    11485..11496

                     /gene="VP24"

                     /locus_tag="ZEBOVgp6"

                     /note="putative"

     misc_feature    11497..11500

                     /gene="VP24"

                     /locus_tag="ZEBOVgp6"

                     /note="intergenic region"

     gene            11501..18282

                     /gene="L"

                     /locus_tag="ZEBOVgp7"

                     /db_xref="GeneID:911824"

     mRNA            11501..18282

                     /gene="L"

                     /locus_tag="ZEBOVgp7"

                     /product="polymerase"

                     /citation=[1]

                     /db_xref="GeneID:911824"

     misc_signal     11501..11512

                     /gene="L"

                     /locus_tag="ZEBOVgp7"

                     /note="transcription start signal"

                     /citation=[1]

     polyA_signal    11508..11518

                     /gene="VP24"

                     /locus_tag="ZEBOVgp6"

     CDS             11581..18219

                     /gene="L"

                     /locus_tag="ZEBOVgp7"

                     /function="synthesis of viral RNAs; transcriptional RNA

                     editing"

                     /note="polymerase"

                     /citation=[1]

                     /codon_start=1

                     /product="RNA-dependent RNA polymerase"

                     /protein_id="NP_066251.1"

                     /db_xref="GI:10313999"

                     /db_xref="GeneID:911824"

                     /translation="MATQHTQYPDARLSSPIVLDQCDLVTRACGLYSSYSLNPQLRNC

                     KLPKHIYRLKYDVTVTKFLSDVPVATLPIDFIVPVLLKALSGNGFCPVEPRCQQFLDE

                     IIKYTMQDALFLKYYLKNVGAQEDCVDEHFQEKILSSIQGNEFLHQMFFWYDLAILTR

                     RGRLNRGNSRSTWFVHDDLIDILGYGDYVFWKIPISMLPLNTQGIPHAAMDWYQASVF

                     KEAVQGHTHIVSVSTADVLIMCKDLITCRFNTTLISKIAEIEDPVCSDYPNFKIVSML

                     YQSGDYLLSILGSDGYKIIKFLEPLCLAKIQLCSKYTERKGRFLTQMHLAVNHTLEEI

                     TEMRALKPSQAQKIREFHRTLIRLEMTPQQLCELFSIQKHWGHPVLHSETAIQKVKKH

                     ATVLKALRPIVIFETYCVFKYSIAKHYFDSQGSWYSVTSDRNLTPGLNSYIKRNQFPP

                     LPMIKELLWEFYHLDHPPLFSTKIISDLSIFIKDRATAVERTCWDAVFEPNVLGYNPP

                     HKFSTKRVPEQFLEQENFSIENVLSYAQKLEYLLPQYRNFSFSLKEKELNVGRTFGKL

                     PYPTRNVQTLCEALLADGLAKAFPSNMMVVTEREQKESLLHQASWHHTSDDFGEHATV

                     RGSSFVTDLEKYNLAFRYEFTAPFIEYCNRCYGVKNVFNWMHYTIPQCYMHVSDYYNP

                     PHNLTLENRDNPPEGPSSYRGHMGGIEGLQQKLWTSISCAQISLVEIKTGFKLRSAVM

                     GDNQCITVLSVFPLETDADEQEQSAEDNAARVAASLAKVTSACGIFLKPDETFVHSGF

                     IYFGKKQYLNGVQLPQSLKTATRMAPLSDAIFDDLQGTLASIGTAFERSISETRHIFP

                     CRITAAFHTFFSVRILQYHHLGFNKGFDLGQLTLGKPLDFGTISLALAVPQVLGGLSF

                     LNPEKCFYRNLGDPVTSGLFQLKTYLRMIEMDDLFLPLIAKNPGNCTAIDFVLNPSGL

                     NVPGSQDLTSFLRQIVRRTITLSAKNKLINTLFHASADFEDEMVCKWLLSSTPVMSRF

                     AADIFSRTPSGKRLQILGYLEGTRTLLASKIINNNTETPVLDRLRKITLQRWSLWFSY

                     LDHCDNILAEALTQITCTVDLAQILREYSWAHILEGRPLIGATLPCMIEQFKVFWLKP

                     YEQCPQCSNAKQPGGKPFVSVAVKKHIVSAWPNASRISWTIGDGIPYIGSRTEDKIGQ

                     PAIKPKCPSAALREAIELASRLTWVTQGSSNSDLLIKPFLEARVNLSVQEILQMTPSH

                     YSGNIVHRYNDQYSPHSFMANRMSNSATRLIVSTNTLGEFSGGGQSARDSNIIFQNVI

                     NYAVALFDIKFRNTEATDIQYNRAHLHLTKCCTREVPAQYLTYTSTLDLDLTRYRENE

                     LIYDSNPLKGGLNCNISFDNPFFQGKRLNIIEDDLIRLPHLSGWELAKTIMQSIISDS

                     NNSSTDPISSGETRSFTTHFLTYPKIGLLYSFGAFVSYYLGNTILRTKKLTLDNFLYY

                     LTTQIHNLPHRSLRILKPTFKHASVMSRLMSIDPHFSIYIGGAAGDRGLSDAARLFLR

                     TSISSFLTFVKEWIINRGTIVPLWIVYPLEGQNPTPVNNFLYQIVELLVHDSSRQQAF

                     KTTISDHVHPHDNLVYTCKSTASNFFHASLAYWRSRHRNSNRKYLARDSSTGSSTNNS

                     DGHIERSQEQTTRDPHDGTERNLVLQMSHEIKRTTIPQENTHQGPSFQSFLSDSACGT

                     ANPKLNFDRSRHNVKFQDHNSASKREGHQIISHRLVLPFFTLSQGTRQLTSSNESQTQ

                     DEISKYLRQLRSVIDTTVYCRFTGIVSSMHYKLDEVLWEIESFKSAVTLAEGEGAGAL

                     LLIQKYQVKTLFFNTLATESSIESEIVSGMTTPRMLLPVMSKFHNDQIEIILNNSASQ

                     ITDITNPTWFKDQRARLPKQVEVITMDAETTENINRSKLYEAVYKLILHHIDPSVLKA

                     VVLKVFLSDTEGMLWLNDNLAPFFATGYLIKPITSSARSSEWYLCLTNFLSTTRKMPH

                     QNHLSCKQVILTALQLQIQRSPYWLSHLTQYADCELHLSYIRLGFPSLEKVLYHRYNL

                     VDSKRGPLVSITQHLAHLRAEIRELTNDYNQQRQSRTQTYHFIRTAKGRITKLVNDYL

                     KFFLIVQALKHNGTWQAEFKKLPELISVCNRFYHIRDCNCEERFLVQTLYLHRMQDSE

                     VKLIERLTGLLSLFPDGLYRFD"

     misc_feature    11608..14853

                     /gene="L"

                     /locus_tag="ZEBOVgp7"

                     /note="Mononegavirales RNA dependent RNA polymerase;

                     Region: Mononeg_RNA_pol; pfam00946"

                     /db_xref="CDD:250248"

     misc_feature    15223..18192

                     /gene="L"

                     /locus_tag="ZEBOVgp7"

                     /note="mRNA capping enzyme, paramyxovirus family; Region:

                     paramyx_RNAcap; TIGR04198"

                     /db_xref="CDD:234496"

     polyA_signal    18272..18282

                     /gene="L"

                     /locus_tag="ZEBOVgp7"

                     /citation=[1]

     3'UTR           18283..18959

                     /note="putative trailer region"

                     /citation=[1]

                     /function="regulation or initiation of RNA replication"

ORIGIN      

        1 cggacacaca aaaagaaaga agaattttta ggatcttttg tgtgcgaata actatgagga

       61 agattaataa ttttcctctc attgaaattt atatcggaat ttaaattgaa attgttactg

      121 taatcacacc tggtttgttt cagagccaca tcacaaagat agagaacaac ctaggtctcc

      181 gaagggagca agggcatcag tgtgctcagt tgaaaatccc ttgtcaacac ctaggtctta

      241 tcacatcaca agttccacct cagactctgc agggtgatcc aacaacctta atagaaacat

      301 tattgttaaa ggacagcatt agttcacagt caaacaagca agattgagaa ttaaccttgg

      361 ttttgaactt gaacacttag gggattgaag attcaacaac cctaaagctt ggggtaaaac

      421 attggaaata gttaaaagac aaattgctcg gaatcacaaa attccgagta tggattctcg

      481 tcctcagaaa atctggatgg cgccgagtct cactgaatct gacatggatt accacaagat

      541 cttgacagca ggtctgtccg ttcaacaggg gattgttcgg caaagagtca tcccagtgta

      601 tcaagtaaac aatcttgaag aaatttgcca acttatcata caggcctttg aagcaggtgt

      661 tgattttcaa gagagtgcgg acagtttcct tctcatgctt tgtcttcatc atgcgtacca

      721 gggagattac aaacttttct tggaaagtgg cgcagtcaag tatttggaag ggcacgggtt

      781 ccgttttgaa gtcaagaagc gtgatggagt gaagcgcctt gaggaattgc tgccagcagt

      841 atctagtgga aaaaacatta agagaacact tgctgccatg ccggaagagg agacaactga

      901 agctaatgcc ggtcagtttc tctcctttgc aagtctattc cttccgaaat tggtagtagg

      961 agaaaaggct tgccttgaga aggttcaaag gcaaattcaa gtacatgcag agcaaggact

     1021 gatacaatat ccaacagctt ggcaatcagt aggacacatg atggtgattt tccgtttgat

     1081 gcgaacaaat tttctgatca aatttctcct aatacaccaa gggatgcaca tggttgccgg

     1141 gcatgatgcc aacgatgctg tgatttcaaa ttcagtggct caagctcgtt tttcaggctt

     1201 attgattgtc aaaacagtac ttgatcatat cctacaaaag acagaacgag gagttcgtct

     1261 ccatcctctt gcaaggaccg ccaaggtaaa aaatgaggtg aactccttta aggctgcact

     1321 cagctccctg gccaagcatg gagagtatgc tcctttcgcc cgacttttga acctttctgg

     1381 agtaaataat cttgagcatg gtcttttccc tcaactatcg gcaattgcac tcggagtcgc

     1441 cacagcacac gggagtaccc tcgcaggagt aaatgttgga gaacagtatc aacaactcag

     1501 agaggctgcc actgaggctg agaagcaact ccaacaatat gcagagtctc gcgaacttga

     1561 ccatcttgga cttgatgatc aggaaaagaa aattcttatg aacttccatc agaaaaagaa

     1621 cgaaatcagc ttccagcaaa caaacgctat ggtaactcta agaaaagagc gcctggccaa

     1681 gctgacagaa gctatcactg ctgcgtcact gcccaaaaca agtggacatt acgatgatga

     1741 tgacgacatt ccctttccag gacccatcaa tgatgacgac aatcctggcc atcaagatga

     1801 tgatccgact gactcacagg atacgaccat tcccgatgtg gtggttgatc ccgatgatgg

     1861 aagctacggc gaataccaga gttactcgga aaacggcatg aatgcaccag atgacttggt

     1921 cctattcgat ctagacgagg acgacgagga cactaagcca gtgcctaata gatcgaccaa

     1981 gggtggacaa cagaagaaca gtcaaaaggg ccagcatata gagggcagac agacacaatc

     2041 caggccaatt caaaatgtcc caggccctca cagaacaatc caccacgcca gtgcgccact

     2101 cacggacaat gacagaagaa atgaaccctc cggctcaacc agccctcgca tgctgacacc

     2161 aattaacgaa gaggcagacc cactggacga tgccgacgac gagacgtcta gccttccgcc

     2221 cttggagtca gatgatgaag agcaggacag ggacggaact tccaaccgca cacccactgt

     2281 cgccccaccg gctcccgtat acagagatca ctctgaaaag aaagaactcc cgcaagacga

     2341 gcaacaagat caggaccaca ctcaagaggc caggaaccag gacagtgaca acacccagtc

     2401 agaacactct tttgaggaga tgtatcgcca cattctaaga tcacaggggc catttgatgc

     2461 tgttttgtat tatcatatga tgaaggatga gcctgtagtt ttcagtacca gtgatggcaa

     2521 agagtacacg tatccagact cccttgaaga ggaatatcca ccatggctca ctgaaaaaga

     2581 ggctatgaat gaagagaata gatttgttac attggatggt caacaatttt attggccggt

     2641 gatgaatcac aagaataaat tcatggcaat cctgcaacat catcagtgaa tgagcatgga

     2701 acaatgggat gattcaaccg acaaatagct aacattaagt agtcaaggaa cgaaaacagg

     2761 aagaattttt gatgtctaag gtgtgaatta ttatcacaat aaaagtgatt cttatttttg

     2821 aatttaaagc tagcttatta ttactagccg tttttcaaag ttcaatttga gtcttaatgc

     2881 aaataggcgt taagccacag ttatagccat aattgtaact caatattcta actagcgatt

     2941 tatctaaatt aaattacatt atgcttttat aacttaccta ctagcctgcc caacatttac

     3001 acgatcgttt tataattaag aaaaaactaa tgatgaagat taaaaccttc atcatcctta

     3061 cgtcaattga attctctagc actcgaagct tattgtcttc aatgtaaaag aaaagctggt

     3121 ctaacaagat gacaactaga acaaagggca ggggccatac tgcggccacg actcaaaacg

     3181 acagaatgcc aggccctgag ctttcgggct ggatctctga gcagctaatg accggaagaa

     3241 ttcctgtaag cgacatcttc tgtgatattg agaacaatcc aggattatgc tacgcatccc

     3301 aaatgcaaca aacgaagcca aacccgaaga cgcgcaacag tcaaacccaa acggacccaa

     3361 tttgcaatca tagttttgag gaggtagtac aaacattggc ttcattggct actgttgtgc

     3421 aacaacaaac catcgcatca gaatcattag aacaacgcat tacgagtctt gagaatggtc

     3481 taaagccagt ttatgatatg gcaaaaacaa tctcctcatt gaacagggtt tgtgctgaga

     3541 tggttgcaaa atatgatctt ctggtgatga caaccggtcg ggcaacagca accgctgcgg

     3601 caactgaggc ttattgggcc gaacatggtc aaccaccacc tggaccatca ctttatgaag

     3661 aaagtgcgat tcggggtaag attgaatcta gagatgagac cgtccctcaa agtgttaggg

     3721 aggcattcaa caatctaaac agtaccactt cactaactga ggaaaatttt gggaaacctg

     3781 acatttcggc aaaggatttg agaaacatta tgtatgatca cttgcctggt tttggaactg

     3841 ctttccacca attagtacaa gtgatttgta aattgggaaa agatagcaac tcattggaca

     3901 tcattcatgc tgagttccag gccagcctgg ctgaaggaga ctctcctcaa tgtgccctaa

     3961 ttcaaattac aaaaagagtt ccaatcttcc aagatgctgc tccacctgtc atccacatcc

     4021 gctctcgagg tgacattccc cgagcttgcc agaaaagctt gcgtccagtc ccaccatcgc

     4081 ccaagattga tcgaggttgg gtatgtgttt ttcagcttca agatggtaaa acacttggac

     4141 tcaaaatttg agccaatctc ccttccctcc gaaagaggcg aataatagca gaggcttcaa

     4201 ctgctgaact atagggtacg ttacattaat gatacacttg tgagtatcag ccctggataa

     4261 tataagtcaa ttaaacgacc aagataaaat tgttcatatc tcgctagcag cttaaaatat

     4321 aaatgtaata ggagctatat ctctgacagt attataatca attgttatta agtaacccaa

     4381 accaaaagtg atgaagatta agaaaaacct acctcggctg agagagtgtt ttttcattaa

     4441 ccttcatctt gtaaacgttg agcaaaattg ttaaaaatat gaggcgggtt atattgccta

     4501 ctgctcctcc tgaatatatg gaggccatat accctgtcag gtcaaattca acaattgcta

     4561 gaggtggcaa cagcaataca ggcttcctga caccggagtc agtcaatggg gacactccat

     4621 cgaatccact caggccaatt gccgatgaca ccatcgacca tgccagccac acaccaggca

     4681 gtgtgtcatc agcattcatc cttgaagcta tggtgaatgt catatcgggc cccaaagtgc

     4741 taatgaagca aattccaatt tggcttcctc taggtgtcgc tgatcaaaag acctacagct

     4801 ttgactcaac tacggccgcc atcatgcttg cttcatacac tatcacccat ttcggcaagg

     4861 caaccaatcc acttgtcaga gtcaatcggc tgggtcctgg aatcccggat catcccctca

     4921 ggctcctgcg aattggaaac caggctttcc tccaggagtt cgttcttccg ccagtccaac

     4981 taccccagta tttcaccttt gatttgacag cactcaaact gatcacccaa ccactgcctg

     5041 ctgcaacatg gaccgatgac actccaacag gatcaaatgg agcgttgcgt ccaggaattt

     5101 catttcatcc aaaacttcgc cccattcttt tacccaacaa aagtgggaag aaggggaaca

     5161 gtgccgatct aacatctccg gagaaaatcc aagcaataat gacttcactc caggacttta

     5221 agatcgttcc aattgatcca accaaaaata tcatgggaat cgaagtgcca gaaactctgg

     5281 tccacaagct gaccggtaag aaggtgactt ctaaaaatgg acaaccaatc atccctgttc

     5341 ttttgccaaa gtacattggg ttggacccgg tggctccagg agacctcacc atggtaatca

     5401 cacaggattg tgacacgtgt cattctcctg caagtcttcc agctgtgatt gagaagtaat

     5461 tgcaataatt gactcagatc cagttttata gaatcttctc agggatagtg ataacatcta

     5521 tttagtaatc cgtccattag aggagacact tttaattgat caatatacta aaggtgcttt

     5581 acaccattgt cttttttctc tcctaaatgt agaacttaac aaaagactca taatatactt

     5641 gtttttaaag gattgattga tgaaagatca taactaataa cattacaaat aatcctacta

     5701 taatcaatac ggtgattcaa atgttaatct ttctcattgc acatactttt tgcccttatc

     5761 ctcaaattgc ctgcatgctt acatctgagg atagccagtg tgacttggat tggaaatgtg

     5821 gagaaaaaat cgggacccat ttctaggttg ttcacaatcc aagtacagac attgcccttc

     5881 taattaagaa aaaatcggcg atgaagatta agccgacagt gagcgtaatc ttcatctctc

     5941 ttagattatt tgttttccag agtaggggtc gtcaggtcct tttcaatcgt gtaaccaaaa

     6001 taaactccac tagaaggata ttgtggggca acaacacaat gggcgttaca ggaatattgc

     6061 agttacctcg tgatcgattc aagaggacat cattctttct ttgggtaatt atccttttcc

     6121 aaagaacatt ttccatccca cttggagtca tccacaatag cacattacag gttagtgatg

     6181 tcgacaaact agtttgtcgt gacaaactgt catccacaaa tcaattgaga tcagttggac

     6241 tgaatctcga agggaatgga gtggcaactg acgtgccatc tgcaactaaa agatggggct

     6301 tcaggtccgg tgtcccacca aaggtggtca attatgaagc tggtgaatgg gctgaaaact

     6361 gctacaatct tgaaatcaaa aaacctgacg ggagtgagtg tctaccagca gcgccagacg

     6421 ggattcgggg cttcccccgg tgccggtatg tgcacaaagt atcaggaacg ggaccgtgtg

     6481 ccggagactt tgccttccat aaagagggtg ctttcttcct gtatgatcga cttgcttcca

     6541 cagttatcta ccgaggaacg actttcgctg aaggtgtcgt tgcatttctg atactgcccc

     6601 aagctaagaa ggacttcttc agctcacacc ccttgagaga gccggtcaat gcaacggagg

     6661 acccgtctag tggctactat tctaccacaa ttagatatca ggctaccggt tttggaacca

     6721 atgagacaga gtacttgttc gaggttgaca atttgaccta cgtccaactt gaatcaagat

     6781 tcacaccaca gtttctgctc cagctgaatg agacaatata tacaagtggg aaaaggagca

     6841 ataccacggg aaaactaatt tggaaggtca accccgaaat tgatacaaca atcggggagt

     6901 gggccttctg ggaaactaaa aaaacctcac tagaaaaatt cgcagtgaag agttgtcttt

     6961 cacagttgta tcaaacggag ccaaaaacat cagtggtcag agtccggcgc gaacttcttc

     7021 cgacccaggg accaacacaa caactgaaga ccacaaaatc atggcttcag aaaattcctc

     7081 tgcaatggtt caagtgcaca gtcaaggaag ggaagctgca gtgtcgcatc taacaaccct

     7141 tgccacaatc tccacgagtc cccaatccct cacaaccaaa ccaggtccgg acaacagcac

     7201 ccataataca cccgtgtata aacttgacat ctctgaggca actcaagttg aacaacatca

     7261 ccgcagaaca gacaacgaca gcacagcctc cgacactccc tctgccacga ccgcagccgg

     7321 acccccaaaa gcagagaaca ccaacacgag caagagcact gacttcctgg accccgccac

     7381 cacaacaagt ccccaaaacc acagcgagac cgctggcaac aacaacactc atcaccaaga

     7441 taccggagaa gagagtgcca gcagcgggaa gctaggctta attaccaata ctattgctgg

     7501 agtcgcagga ctgatcacag gcgggagaag aactcgaaga gaagcaattg tcaatgctca

     7561 acccaaatgc aaccctaatt tacattactg gactactcag gatgaaggtg ctgcaatcgg

     7621 actggcctgg ataccatatt tcgggccagc agccgaggga atttacatag aggggctaat

     7681 gcacaatcaa gatggtttaa tctgtgggtt gagacagctg gccaacgaga cgactcaagc

     7741 tcttcaactg ttcctgagag ccacaactga gctacgcacc ttttcaatcc tcaaccgtaa

     7801 ggcaattgat ttcttgctgc agcgatgggg cggcacatgc cacattctgg gaccggactg

     7861 ctgtatcgaa ccacatgatt ggaccaagaa cataacagac aaaattgatc agattattca

     7921 tgattttgtt gataaaaccc ttccggacca gggggacaat gacaattggt ggacaggatg

     7981 gagacaatgg ataccggcag gtattggagt tacaggcgtt ataattgcag ttatcgcttt

     8041 attctgtata tgcaaatttg tcttttagtt tttcttcaga ttgcttcatg gaaaagctca

     8101 gcctcaaatc aatgaaacca ggatttaatt atatggatta cttgaatcta agattacttg

     8161 acaaatgata atataataca ctggagcttt aaacatagcc aatgtgattc taactccttt

     8221 aaactcacag ttaatcataa acaaggtttg acatcaatct agttatctct ttgagaatga

     8281 taaacttgat gaagattaag aaaaaggtaa tctttcgatt atctttaatc ttcatccttg

     8341 attctacaat catgacagtt gtctttagtg acaagggaaa gaagcctttt tattaagttg

     8401 taataatcag atctgcgaac cggtagagtt tagttgcaac ctaacacaca taaagcattg

     8461 gtcaaaaagt caatagaaat ttaaacagtg agtggagaca acttttaaat ggaagcttca

     8521 tatgagagag gacgcccacg agctgccaga cagcattcaa gggatggaca cgaccaccat

     8581 gttcgagcac gatcatcatc cagagagaat tatcgaggtg agtaccgtca atcaaggagc

     8641 gcctcacaag tgcgcgttcc tactgtattt cataagaaga gagttgaacc attaacagtt

     8701 cctccagcac ctaaagacat atgtccgacc ttgaaaaaag gatttttgtg tgacagtagt

     8761 ttttgcaaaa aagatcacca gttggagagt ttaactgata gggaattact cctactaatc

     8821 gcccgtaaga cttgtggatc agtagaacaa caattaaata taactgcacc caaggactcg

     8881 cgcttagcaa atccaacggc tgatgatttc cagcaagagg aaggtccaaa aattaccttg

     8941 ttgacactga tcaagacggc agaacactgg gcgagacaag acatcagaac catagaggat

     9001 tcaaaattaa gagcattgtt gactctatgt gctgtgatga cgaggaaatt ctcaaaatcc

     9061 cagctgagtc ttttatgtga gacacaccta aggcgcgagg ggcttgggca agatcaggca

     9121 gaacccgttc tcgaagtata tcaacgatta cacagtgata aaggaggcag ttttgaagct

     9181 gcactatggc aacaatggga ccgacaatcc ctaattatgt ttatcactgc attcttgaat

     9241 attgctctcc agttaccgtg tgaaagttct gctgtcgttg tttcagggtt aagaacattg

     9301 gttcctcaat cagataatga ggaagcttca accaacccgg ggacatgctc atggtctgat

     9361 gagggtaccc cttaataagg ctgactaaaa cactatataa ccttctactt gatcacaata

     9421 ctccgtatac ctatcatcat atatttaatc aagacgatat cctttaaaac ttattcagta

     9481 ctataatcac tctcgtttca aattaataag atgtgcatga ttgccctaat atatgaagag

     9541 gtatgataca accctaacag tgatcaaaga aaatcataat ctcgtatcgc tcgtaatata

     9601 acctgccaag catacctctt gcacaaagtg attcttgtac acaaataatg ttttactcta

     9661 caggaggtag caacgatcca tcccatcaaa aaataagtat ttcatgactt actaatgatc

     9721 tcttaaaata ttaagaaaaa ctgacggaac ataaattctt tatgcttcaa gctgtggagg

     9781 aggtgtttgg tattggctat tgttatatta caatcaataa caagcttgta aaaatattgt

     9841 tcttgtttca agaggtagat tgtgaccgga aatgctaaac taatgatgaa gattaatgcg

     9901 gaggtctgat aagaataaac cttattattc agattaggcc ccaagaggca ttcttcatct

     9961 ccttttagca aagtactatt tcagggtagt ccaattagtg gcacgtcttt tagctgtata

    10021 tcagtcgccc ctgagatacg ccacaaaagt gtctctaagc taaattggtc tgtacacatc

    10081 ccatacattg tattaggggc aataatatct aattgaactt agccgtttaa aatttagtgc

    10141 ataaatctgg gctaacacca ccaggtcaac tccattggct gaaaagaagc ttacctacaa

    10201 cgaacatcac tttgagcgcc ctcacaatta aaaaatagga acgtcgttcc aacaatcgag

    10261 cgcaaggttt caaggttgaa ctgagagtgt ctagacaaca aaatattgat actccagaca

    10321 ccaagcaaga cctgagaaaa aaccatggct aaagctacgg gacgatacaa tctaatatcg

    10381 cccaaaaagg acctggagaa aggggttgtc ttaagcgacc tctgtaactt cttagttagc

    10441 caaactattc aggggtggaa ggtttattgg gctggtattg agtttgatgt gactcacaaa

    10501 ggaatggccc tattgcatag actgaaaact aatgactttg cccctgcatg gtcaatgaca

    10561 aggaatctct ttcctcattt atttcaaaat ccgaattcca caattgaatc accgctgtgg

    10621 gcattgagag tcatccttgc agcagggata caggaccagc tgattgacca gtctttgatt

    10681 gaacccttag caggagccct tggtctgatc tctgattggc tgctaacaac caacactaac

    10741 catttcaaca tgcgaacaca acgtgtcaag gaacaattga gcctaaaaat gctgtcgttg

    10801 attcgatcca atattctcaa gtttattaac aaattggatg ctctacatgt cgtgaactac

    10861 aacggattgt tgagcagtat tgaaattgga actcaaaatc atacaatcat cataactcga

    10921 actaacatgg gttttctggt ggagctccaa gaacccgaca aatcggcaat gaaccgcatg

    10981 aagcctgggc cggcgaaatt ttccctcctt catgagtcca cactgaaagc atttacacaa

    11041 ggatcctcga cacgaatgca aagtttgatt cttgaattta atagctctct tgctatctaa

    11101 ctaaggtaga atacttcata ttgagctaac tcatatatgc tgactcaata gttatcttga

    11161 catctctgct ttcataatca gatatataag cataataaat aaatactcat atttcttgat

    11221 aatttgttta accacagata aatcctcact gtaagccagc ttccaagttg acacccttac

    11281 aaaaaccagg actcagaatc cctcaaacaa gagattccaa gacaacatca tagaattgct

    11341 ttattatatg aataagcatt ttatcaccag aaatcctata tactaaatgg ttaattgtaa

    11401 ctgaacccgc aggtcacatg tgttaggttt cacagattct atatattact aactctatac

    11461 tcgtaattaa cattagataa gtagattaag aaaaaagcct gaggaagatt aagaaaaact

    11521 gcttattggg tctttccgtg ttttagatga agcagttgaa attcttcctc ttgatattaa

    11581 atggctacac aacataccca atacccagac gctaggttat catcaccaat tgtattggac

    11641 caatgtgacc tagtcactag agcttgcggg ttatattcat catactccct taatccgcaa

    11701 ctacgcaact gtaaactccc gaaacatatc taccgtttga aatacgatgt aactgttacc

    11761 aagttcttga gtgatgtacc agtggcgaca ttgcccatag atttcatagt cccagttctt

    11821 ctcaaggcac tgtcaggcaa tggattctgt cctgttgagc cgcggtgcca acagttctta

    11881 gatgaaatca ttaagtacac aatgcaagat gctctcttct tgaaatatta tctcaaaaat

    11941 gtgggtgctc aagaagactg tgttgatgaa cactttcaag agaaaatctt atcttcaatt

    12001 cagggcaatg aatttttaca tcaaatgttt ttctggtatg atctggctat tttaactcga

    12061 aggggtagat taaatcgagg aaactctaga tcaacatggt ttgttcatga tgatttaata

    12121 gacatcttag gctatgggga ctatgttttt tggaagatcc caatttcaat gttaccactg

    12181 aacacacaag gaatccccca tgctgctatg gactggtatc aggcatcagt attcaaagaa

    12241 gcggttcaag ggcatacaca cattgtttct gtttctactg ccgacgtctt gataatgtgc

    12301 aaagatttaa ttacatgtcg attcaacaca actctaatct caaaaatagc agagattgag

    12361 gatccagttt gttctgatta tcccaatttt aagattgtgt ctatgcttta ccagagcgga

    12421 gattacttac tctccatatt agggtctgat gggtataaaa ttattaagtt cctcgaacca

    12481 ttgtgcttgg ccaaaattca attatgctca aagtacactg agaggaaggg ccgattctta

    12541 acacaaatgc atttagctgt aaatcacacc ctagaagaaa ttacagaaat gcgtgcacta

    12601 aagccttcac aggctcaaaa gatccgtgaa ttccatagaa cattgataag gctggagatg

    12661 acgccacaac aactttgtga gctattttcc attcaaaaac actgggggca tcctgtgcta

    12721 catagtgaaa cagcaatcca aaaagttaaa aaacatgcta cggtgctaaa agcattacgc

    12781 cctatagtga ttttcgagac atactgtgtt tttaaatata gtattgccaa acattatttt

    12841 gatagtcaag gatcttggta cagtgttact tcagatagga atctaacacc gggtcttaat

    12901 tcttatatca aaagaaatca attccctccg ttgccaatga ttaaagaact actatgggaa

    12961 ttttaccacc ttgaccaccc tccacttttc tcaaccaaaa ttattagtga cttaagtatt

    13021 tttataaaag acagagctac cgcagtagaa aggacatgct gggatgcagt attcgagcct

    13081 aatgttctag gatataatcc acctcacaaa tttagtacta aacgtgtacc ggaacaattt

    13141 ttagagcaag aaaacttttc tattgagaat gttctttcct acgcacaaaa actcgagtat

    13201 ctactaccac aatatcggaa cttttctttc tcattgaaag agaaagagtt gaatgtaggt

    13261 agaaccttcg gaaaattgcc ttatccgact cgcaatgttc aaacactttg tgaagctctg

    13321 ttagctgatg gtcttgctaa agcatttcct agcaatatga tggtagttac ggaacgtgag

    13381 caaaaagaaa gcttattgca tcaagcatca tggcaccaca caagtgatga ttttggtgaa

    13441 catgccacag ttagagggag tagctttgta actgatttag agaaatacaa tcttgcattt

    13501 agatatgagt ttacagcacc ttttatagaa tattgcaacc gttgctatgg tgttaagaat

    13561 gtttttaatt ggatgcatta tacaatccca cagtgttata tgcatgtcag tgattattat

    13621 aatccaccac ataacctcac actggagaat cgagacaacc cccccgaagg gcctagttca

    13681 tacaggggtc atatgggagg gattgaagga ctgcaacaaa aactctggac aagtatttca

    13741 tgtgctcaaa tttctttagt tgaaattaag actggtttta agttacgctc agctgtgatg

    13801 ggtgacaatc agtgcattac tgttttatca gtcttcccct tagagactga cgcagacgag

    13861 caggaacaga gcgccgaaga caatgcagcg agggtggccg ccagcctagc aaaagttaca

    13921 agtgcctgtg gaatcttttt aaaacctgat gaaacatttg tacattcagg ttttatctat

    13981 tttggaaaaa aacaatattt gaatggggtc caattgcctc agtcccttaa aacggctaca

    14041 agaatggcac cattgtctga tgcaattttt gatgatcttc aagggaccct ggctagtata

    14101 ggcactgctt ttgagcgatc catctctgag acacgacata tctttccttg caggataacc

    14161 gcagctttcc atacgttttt ttcggtgaga atcttgcaat atcatcatct cgggttcaat

    14221 aaaggttttg accttggaca gttaacactc ggcaaacctc tggatttcgg aacaatatca

    14281 ttggcactag cggtaccgca ggtgcttgga gggttatcct tcttgaatcc tgagaaatgt

    14341 ttctaccgga atctaggaga tccagttacc tcaggcttat tccagttaaa aacttatctc

    14401 cgaatgattg agatggatga tttattctta cctttaattg cgaagaaccc tgggaactgc

    14461 actgccattg actttgtgct aaatcctagc ggattaaatg tccctgggtc gcaagactta

    14521 acttcatttc tgcgccagat tgtacgcagg accatcaccc taagtgcgaa aaacaaactt

    14581 attaatacct tatttcatgc gtcagctgac ttcgaagacg aaatggtttg taaatggcta

    14641 ttatcatcaa ctcctgttat gagtcgtttt gcggccgata tcttttcacg cacgccgagc

    14701 gggaagcgat tgcaaattct aggatacctg gaaggaacac gcacattatt agcctctaag

    14761 atcatcaaca ataatacaga gacaccggtt ttggacagac tgaggaaaat aacattgcaa

    14821 aggtggagcc tatggtttag ttatcttgat cattgtgata atatcctggc ggaggcttta

    14881 acccaaataa cttgcacagt tgatttagca cagattctga gggaatattc atgggctcat

    14941 attttagagg gaagacctct tattggagcc acactcccat gtatgattga gcaattcaaa

    15001 gtgttttggc tgaaacccta cgaacaatgt ccgcagtgtt caaatgcaaa gcaaccaggt

    15061 gggaaaccat tcgtgtcagt ggcagtcaag aaacatattg ttagtgcatg gccgaacgca

    15121 tcccgaataa gctggactat cggggatgga atcccataca ttggatcaag gacagaagat

    15181 aagataggac aacctgctat taaaccaaaa tgtccttccg cagccttaag agaggccatt

    15241 gaattggcgt cccgtttaac atgggtaact caaggcagtt cgaacagtga cttgctaata

    15301 aaaccatttt tggaagcacg agtaaattta agtgttcaag aaatacttca aatgacccct

    15361 tcacattact caggaaatat tgttcacagg tacaacgatc aatacagtcc tcattctttc

    15421 atggccaatc gtatgagtaa ttcagcaacg cgattgattg tttctacaaa cactttaggt

    15481 gagttttcag gaggtggcca gtctgcacgc gacagcaata ttattttcca gaatgttata

    15541 aattatgcag ttgcactgtt cgatattaaa tttagaaaca ctgaggctac agatatccaa

    15601 tataatcgtg ctcaccttca tctaactaag tgttgcaccc gggaagtacc agctcagtat

    15661 ttaacataca catctacatt ggatttagat ttaacaagat accgagaaaa cgaattgatt

    15721 tatgacagta atcctctaaa aggaggactc aattgcaata tctcattcga taatccattt

    15781 ttccaaggta aacggctgaa cattatagaa gatgatctta ttcgactgcc tcacttatct

    15841 ggatgggagc tagccaagac catcatgcaa tcaattattt cagatagcaa caattcatct

    15901 acagacccaa ttagcagtgg agaaacaaga tcattcacta cccatttctt aacttatccc

    15961 aagataggac ttctgtacag ttttggggcc tttgtaagtt attatcttgg caatacaatt

    16021 cttcggacta agaaattaac acttgacaat tttttatatt acttaactac tcaaattcat

    16081 aatctaccac atcgctcatt gcgaatactt aagccaacat tcaaacatgc aagcgttatg

    16141 tcacggttaa tgagtattga tcctcatttt tctatttaca taggcggtgc tgcaggtgac

    16201 agaggactct cagatgcggc caggttattt ttgagaacgt ccatttcatc ttttcttaca

    16261 tttgtaaaag aatggataat taatcgcgga acaattgtcc ctttatggat agtatatccg

    16321 ctagagggtc aaaacccaac acctgtgaat aattttctct atcagatcgt agaactgctg

    16381 gtgcatgatt catcaagaca acaggctttt aaaactacca taagtgatca tgtacatcct

    16441 cacgacaatc ttgtttacac atgtaagagt acagccagca atttcttcca tgcatcattg

    16501 gcgtactgga ggagcagaca cagaaacagc aaccgaaaat acttggcaag agactcttca

    16561 actggatcaa gcacaaacaa cagtgatggt catattgaga gaagtcaaga acaaaccacc

    16621 agagatccac atgatggcac tgaacggaat ctagtcctac aaatgagcca tgaaataaaa

    16681 agaacgacaa ttccacaaga aaacacgcac cagggtccgt cgttccagtc ctttctaagt

    16741 gactctgctt gtggtacagc aaatccaaaa ctaaatttcg atcgatcgag acacaatgtg

    16801 aaatttcagg atcataactc ggcatccaag agggaaggtc atcaaataat ctcacaccgt

    16861 ctagtcctac ctttctttac attatctcaa gggacacgcc aattaacgtc atccaatgag

    16921 tcacaaaccc aagacgagat atcaaagtac ttacggcaat tgagatccgt cattgatacc

    16981 acagtttatt gtagatttac cggtatagtc tcgtccatgc attacaaact tgatgaggtc

    17041 ctttgggaaa tagagagttt caagtcggct gtgacgctag cagagggaga aggtgctggt

    17101 gccttactat tgattcagaa ataccaagtt aagaccttat ttttcaacac gctagctact

    17161 gagtccagta tagagtcaga aatagtatca ggaatgacta ctcctaggat gcttctacct

    17221 gttatgtcaa aattccataa tgaccaaatt gagattattc ttaacaactc agcaagccaa

    17281 ataacagaca taacaaatcc tacttggttt aaagaccaaa gagcaaggct acctaagcaa

    17341 gtcgaggtta taaccatgga tgcagagaca acagagaata taaacagatc gaaattgtac

    17401 gaagctgtat ataaattgat cttacaccat attgatccta gcgtattgaa agcagtggtc

    17461 cttaaagtct ttctaagtga tactgagggt atgttatggc taaatgataa tttagccccg

    17521 ttttttgcca ctggttattt aattaagcca ataacgtcaa gtgctagatc tagtgagtgg

    17581 tatctttgtc tgacgaactt cttatcaact acacgtaaga tgccacacca aaaccatctc

    17641 agttgtaaac aggtaatact tacggcattg caactgcaaa ttcaacgaag cccatactgg

    17701 ctaagtcatt taactcagta tgctgactgt gagttacatt taagttatat ccgccttggt

    17761 tttccatcat tagagaaagt actataccac aggtataacc tcgtcgattc aaaaagaggt

    17821 ccactagtct ctatcactca gcacttagca catcttagag cagagattcg agaattaact

    17881 aatgattata atcaacagcg acaaagtcgg actcaaacat atcactttat tcgtactgca

    17941 aaaggacgaa tcacaaaact agtcaatgat tatttaaaat tctttcttat tgtgcaagca

    18001 ttaaaacata atgggacatg gcaagctgag tttaagaaat taccagagtt gattagtgtg

    18061 tgcaataggt tctaccatat tagagattgc aattgtgaag aacgtttctt agttcaaacc

    18121 ttatatttac atagaatgca ggattctgaa gttaagctta tcgaaaggct gacagggctt

    18181 ctgagtttat ttccggatgg tctctacagg tttgattgaa ttaccgtgca tagtatcctg

    18241 atacttgcaa aggttggtta ttaacataca gattataaaa aactcataaa ttgctctcat

    18301 acatcatatt gatctaatct caataaacaa ctatttaaat aacgaaagga gtccctatat

    18361 tatatactat atttagcctc tctccctgcg tgataatcaa aaaattcaca atgcagcatg

    18421 tgtgacatat tactgccgca atgaatttaa cgcaacataa taaactctgc actctttata

    18481 attaagcttt aacgaaaggt ctgggctcat attgttattg atataataat gttgtatcaa

    18541 tatcctgtca gatggaatag tgttttggtt gataacacaa cttcttaaaa caaaattgat

    18601 ctttaagatt aagtttttta taattatcat tactttaatt tgtcgtttta aaaacggtga

    18661 tagccttaat ctttgtgtaa aataagagat taggtgtaat aaccttaaca tttttgtcta

    18721 gtaagctact atttcataca gaatgataaa attaaaagaa aaggcaggac tgtaaaatca

    18781 gaaatacctt ctttacaata tagcagacta gataataatc ttcgtgttaa tgataattaa

    18841 gacattgacc acgctcatca gaaggctcgc cagaataaac gttgcaaaaa ggattcctgg

    18901 aaaaatggtc gcacacaaaa atttaaaaat aaatctattt cttctttttt gtgtgtcca

//