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Custom siRNA Synthesis - Road to gene silencing

Our custom siRNA synthesis services are delivered in desalted, purified, annealed duplex format with modified nucleotides of DNA , 2'OMe, and LNA bases. Provide your siRNA sequence of choice, or use our design tool to select an siRNA sequence. siRNA duplexes are provided as a lyophilized pellet to ensure stability during delivery. siRNA duplex are available from milligram to gram scale both as chemically stabilized and as unmodified RNA. Large-scale sterile, endotoxin-free preparations are available specifically for in vivo research needs. Contact us for details.

Duplex siRNA - 21-mer

Our siRNA oligos are available at a fixed price per oligonucleotide which are made of 19 RNA bases plus 2 DNA bases 3' overhang. Contact us for longer oligos or spike oligos which are available upon requests.

Desalted			HPLC			In-Vivo-Ready			In-Vivo-Ready HPCL
Yields* (ODs)	Yields* (nmols)	Price	Yields* (ODs)	Yields* (nmols)	Price	Yields* (ODs)	Yields* (nmols)	Price	Yields* (ODs)	Yields* (nmols)	Price
4	20	$210	4 OD	20	$390	20	100	$680	20	100	$1,000
8	40	$295	8 OD	40	$450	50	250	$880	50	250	$1,475
30	150	$390	52 OD	160	$900	200	1000	$1,050	200	1000	$2,325
60	300	$590
150	750	$810

Contact us for large scale siRNA
*Guaranteed yields (ODs) and approximate yields (nmols) are for oligos > 20 bases and < 40 bases *

Duplex siRNA - 27-mer

These siRNA molecules have no dTdT overhang or any sticky overhang at all. 27-mers might show higher efficiency in comparison to the 'classical' 21-mer siRNA in certain cases (Kim et al. Nature Biotechnology 2005 23 (2):222-226).

Desalted			HPLC			In-Vivo-Ready			In-Vivo-Ready HPCL
Yields* (ODs)	Yields* (nmols)	Price	Yields* (ODs)	Yields* (nmols)	Price	Yields* (ODs)	Yields* (nmols)	Price	Yields* (ODs)	Yields* (nmols)	Price
4	20	$360	4 OD	20	$440	20	100	$780	20	100	$1,300
8	40	$340	8 OD	40	$500	50	250	$980	50	250	$1,675
30	150	$430	52 OD	160	$950	200	1000	$1,350	200	1000	$2,725
60	300	$630
150	750	$850

Contact us for large scale siRNA
*Guaranteed yields (ODs) and approximate yields (nmols) are for oligos > 20 bases and < 40 bases *

Sample Processing Options

Desalted Single-Stranded:

1. The oligos are shipped in the deprotected, desalted single strand in a separate tube.
2. The dried duplex is provided in the absence of universal buffer salts.
3. The oligos are ready for use immediately upon resuspension.
4. The expected ship date is 4 business days for orders received before 3:00pm CST.

Desalted Duplex:

1. The oligos are shipped in the deprotected, desalted duplex form.
2. The dried duplex is provided in the absence of universal buffer salts.
3. The oligos are ready for use immediately upon resuspension.
4. The expected ship date is 4 business days for orders received before 3:00pm CST.

HPLC purified: *Special Note: The siRNA duplex is now dried down in the absence of buffer and the amount packaged per tube has changed, however, the total amount of material supplied remains the same.

1. The oligos are shipped in the deprotected, duplexed, purified, and desalted form.
2. The dried duplex is provided in the absence of universal buffer salts, unless otherwise specified upon ordering.
3. The oligos are ready for use immediately upon resuspension.
4. The expected ship date is 6 business days for orders received before 3:00pm CST.

siRNA In-Vivo-Ready: A minimum of 90% purity. These duplexes are shipped in the deprotected form with extra processing also including counter ion (Na+) exchange, dialysis, sterile filtration and endotoxin testing for the introduction of siRNAs into animals.

The estimated ship date is 10 business days for orders received before 3:00pm CST. Ship date will be confirmed upon order placement.
siRNA In-Vivo-Ready HPLC: This In-Vivo-Ready custom synthesized siRNAs provide the highest quality siRNAs available anywhere ith guaranteed purity of 97%. The processing of the oligos includes extra level of HPLC purification, counter ion (Na+) exchange, dialysis to remove salt (to <1 microsiemens conductance at 50 µM siRNA), sterile filtration and endotoxin tested.

The estimated ship date is 20 business days for orders received before 3:00pm CST. Ship date will be confirmed upon order placement.

Quality Control Options

Standard QC package

Analytical anion-exchange HPLC of oligonucleotide; purity guaranteed above 85% in case purification is required. TOF/MS for oligonucleotide identification.

Advanced QC package

Standard QC package and additionally:

Ion-pair reverse phase HPLC of the oligonucleotide at 60 ^oC, purity guaranteed above 85% analytical reverse phase HPLC Endotoxin analysis of the oligonucleotide (*phosphorothioates may inhibit in the mechanism for endotoxin determinations Bio- Synthesis is using and therefore reading might be less accurate for these molecules) pH determination of a 1% oligonucleotide solution.

Custom QC Package: Custom QC package such as:

Determination of the sodium content of the oligonucleotide (minimum 20 mg of purified oligonucleotide is required). LC-MS of the oligonucleotides. Any custom quality control methods could be developed. Our technical service staffs are willing to respond to your various advices and requests.

The most trusted name in Quality Custom Synthesis