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Large scale oligonucleotide synthesis

From research to drug discovery...

15-25 bases
50mg to 10 grams
Oligo modifications available
DNA, RNA, LNA, PNA
cGMP and QSR manufacturing available

Using a new synthesis technology, Bio-Synthesis is able to meet our customer requirements for high quality DNA, RNA, LNA, PNA oligonucleotide from mg to multi-gram quantity for pre-clinical, diagnostic or therapeutic studies. At Bio-Synthesis, DNA, RNA, LNA, PNA can be synthesized at mid-scale range from mg to multi-gram quantity and maintaining our quality commitment. All products are analyzed by PAGE, HPLC and mass spectroscopy. Turn around time varies based on sequence, purification and quantity requested. It is always advisable to order your material well in advance to ensure that you receive the material prior to establishing your studies.

Synthesis

Use of dedicated equipment for large-scale synthesis enables synthesis of gram quantity oligonucleotide (under 25 mers) per one lot. All the products have their original lot numbers and are under the strict lot control.

Backbone Options

Backbone	PO	PS
DNA
2' OH RNA
2' OMe RNA
Methylphosphoate	n/a	n/a
2' Fluoro RNA (C & U)
Lock Nucleic Acid
Peptide Nucleic Acid	n/a	n/a

Purification

Several types purification setting as default

Standard Purification
Salt content is as important as purity in large-scale synthesis. This is the reason for setting RP-Cartridge and desalting as a default purification method for this service.

HPLC Purification

HPLC is well suited to purify any modified oligo or unmodified sequences up to 60 bases in length and is the method of choice for purifying larger amounts of oligos (i.e. > 1 mol). Purity of 85% is guaranteed for oligo upto 40 bases.
Ion-exchange HPLC (IE-HPLC) is available to purify long oligos up to 60 bases. Anion-exchange resins separate oligos on the basis of charge. The resolution of this technique is very high but their use is limited to purification of rather short oligos. For unmodified oligos between 11-40, purity of > 90% is guaranteed.
Dual HPLC Purification and Dual PAGE and HPLC Purification a combined purifications are applied for this service: HPLC + IEX HPLC purifications for 20-30 mers oligos, and HPLC + PAGE purifications for 31 - 50 mers oligos. Such combined purifications enable averages of 98% purity by taking off most of base defective products or other impurities.
RNAase Free HPLC Purifications Originally developed for isolating RNA oligos, RNase-Free HPLC Purification has been extended to DNA oligos for use when there is high senstivity to ribonucleases. RNase-Free HPLC purification is carefully performed in an RNase-Free environment; reagents, equipment and lab surfaces are monitored for RNase contamination.

Vivo-Ready Purification
Bio-Synthesis's In Vivo-Ready purification option provides high quality oligos that are ready for introduction into animals. Each oligo is HPLC purified, dialyzed to ensure that the oligonucleotide is salt free (conductance of <1 microsiemens for a 50 um solution), sterile filtered, and endotoxin tested. Of course, all oligonucleotide are been anaylzed by MALDI-TOF mass spectrometry and analytical HPLC, and sequencing.

Quality Control

Several types quality control

Standard QC package

Analytical anion-exchange HPLC of oligonucleotide; purity guaranteed above 85% in case purification is required.
TOF/MS for oligonucleotide identification.

Advanced QC package

Standard QC package and additionally:
Ion-pair reverse phase HPLC of the oligonucleotide at 60 C, purity guaranteed above 85%
analytical reverse phase HPLC
Endotoxin analysis of the oligonucleotide (*phosphorothioates may inhibit in the mechanism for endotoxin determinations Bio-Synthesis is using and therefore reading might be less accurate for these molecules)
pH determination of a 1% oligonucleotide solution.

Custom QC Package:

Determination of the sodium content of the oligonucleotide (minimum 20 mg of purified oligonucleotide is required).
LC-MS of the oligonucleotides
Any Custom quality control methods could be developed.

Our technical service staffs are willing to respond to your various advices and requests.

Manufacturing Line Complaint with GMP

Reliable products can be provided by synthesizing in the manufacturing line compliant with GMP (Good Manufacturing Practice). We will support your R&D with stable clean quality.

Pricing

Prices are for unmodified oligonucleotides between 15 and 25 bases. It includes purification and ethanol precipitation. Please request a quotation for other lengths or larger quantities. The backbone may consists of phosphorothioate or phosphodiester linkages, or a combination of the two. Prices may very due to sequence composition.

Please submit your order or quotation request to the client relations department via e-mail, fax (972) 420-0442, or you can use our online ordering system.

The most trusted name in Quality Custom Synthesis