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What is peptide phosphorylation

What is peptide phosphorylation?

Peptide phosphorylation is a peptide containing phosphate (PO43-) group to a peptide containing serine, threonine or tyrosine. Phosphorylation on these amino acids is an extremely important modulator of post-translational modfication of protein function, and is associated with many proteins that have a regulatory function in cells.Therefore, there is a great need for methods capable of accurately elucidating sites of phosphorylation. Protein phosphorylation can occur on several amino acids. Phosphorylation on serine is the most common, followed by threonine. Tyrosine phosphorylation is relatively rare but it is at the origin of protein phosphorylation signaling pathways in most eukaryotes.

Phospho-specific antibody generated by using phosphorylated peptide are commonly used to detect whether a protein is phosphorylated at a particular site. We offer peptide phosphorylation synthesis on pSer, pTyr, or D-pSer, D-pTyr, D-pThr. We can also place phosphorylation site at multiple positions. Although multiple phosphorylated amino acids can be added, they can negatively affect peptide synthesis and purification.

Phoshorylated amino acids we use are:
peptide phosphorylation