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Custom Glycopeptide Synthesis

Does Bio-Synthesis Provide Glycopeptide?

Bio-Synthesis provides glycopeptide synthesis at various scale and purity. These synthetic glycopeptides offer a unique tool in glycobiology and proteomic research as well as for drug discovery and development. We offer different glycoamino acid peptide modification with N-linked and O-linked glycosylation system.

Synthetic N-Linked Glycopeptides

N-linked glycosylation is a post translational process that occurs in eukaryotes and widely in archaea, but is rare in bacteria. It’s now understood that N-glycosylation plays a role in the folding of many eukaryotic proteins. Thus, approximately 70% of human therapeutic proteins are N-linked glycoproteins which contributes to increased in vivo half-life and bioavailability and hence higher therapeutic efficacy.

Synthetic O-Linked Glycopeptides

Oxygen-linked glycopeptides and proteins are quite prevalent. Cell surface and extracellular proteins are O-glycosylated. The most abundant type of O-glycosylation in proteins is the N-acetylgalactosamine or alpha-GalNAc attachment to Ser or Thr in the protein chain. Most eukaryotic nuclear and cytoplasmic proteins are modified by N-acetylglucosamine (beta-O-GlcNAc) linked to Ser or Thr. Less prevalent types of O-glycosylation involve O-fucose (Fuc), O-mannose (Man), and O-glucose (Glc), however they are functionally of high relevance for early stages of development and for vital physiological functions of proteins. Glycosaminoglycans (GAG) are linked to proteoglycans via xylose (Xyl) linked to Ser.

Glycoamino acids we can incorporate into peptides or oligonucleotides are:

Asparagine glycoamino acids
  • N-Link Monosaccharide Asparagine
  • N-Link Disaccharide Asparagine
Serine glycoamino acids
  • Peracetylated O-Link Monosaccharide Serine
  • O-Link Monosaccharide Serine
  • Peracetylated O-Link Disaccharide Serine
  • O-Link Disaccharide Serine
  • O-Link Trisaccharide Serine
  • Peracetylated Core 3-Serine, O-Link Disaccharide Serine
  • Ser(α-D-ManNAc(Ac)3)-OH, O-Link Monosaccharide Serine
  • Ser(β-D-Gal(Ac)4)-OH, O-link Monosaccharide Serine
  • Ser(α-D-Gal(Ac)4)-OH, O-Link Monosaccharide Serine
  • Ser(α-D-Man(Ac)4)-OH, O-Link Monosaccharide Serine
  • Ser(β-GlcNAc-(1-2)-α-Man)-OH, peracetate, O-Link Disaccharide Serine
  • Ser(α-Fuc(Ac3))-OH, O-Link Monosaccharide Serine
  • Ser(β-Fuc(Ac3))-OH, O-Link Monosaccharide Serine
  • Ser(β-Xyl(Ac3))-OH, O-Link Monosaccharide Serine
  • Ser(β-D-Lac(Ac)7)-OH, O-link Disaccharide Serine
  • Ser(β-D-Cel(Ac)7)-OH, O-Link Disaccharide Serine
  • Ser(β-D-Mel(Ac)7)-OH, O-Link Disaccharide Serine
  • Ser(β-D-Mal(Ac)7)-OH, O-Link Disaccharide Serine
Threonine Glycoamino Acids
  • Peracetylated Threonine Thr(α-D-GalNAc(Ac)3)-OH, O-Link Monosaccharide Threonine
  • Thr(β-D-GalNAc(Ac)3)-OH, O-Link Monosaccharide Threonine
  • Peracetylated Thr(Gal β(1-3)GalNAc)-OH, O-Link Disaccharide Threonine
  • Peracetylated, Methyl ester, Thr(α-Neu5Ac (2-6)-α-GalNAc)-OH, O-Link Disaccharide Threonine
  • Peracetylated, Methyl ester, Thr(α-Neu5Ac-(2-3)-β-Gal-(1-3)-α-GalNAc)-OH, O-Link Trisaccharide Threonine
  • Peracetylated Threonine, Thr(β-GlcNAc-(1-3)-α-GalNAc)-OH, O-Link Disaccharide Threonine
  • Peracetylated Threonine, Core 2 O-Link Trisaccharide Threonine
  • Peracetylated Core 4, O-Link Trisaccharide Threonine
  • Thr(β-D-GlcNAc(Ac)3)-OH, O-Link Monosaccharide Threonine
  • Thr(α-D-ManNAc(Ac)3)-OH, O-Link Monosaccharide Threonine
  • Thr(β-D-Gal(Ac)4)-OH, O-Link Monosaccharide Threonine
  • Thr(α-D-Gal(Ac)4)-OH, O-Link Monosaccharide Threonine
  • Thr(β-D-Glc(Ac)4)-OH, O-Link Monosaccharide Threonine
  • Thr(α-D-Glc(Ac)4)-OH, O-Link Monosaccharide Threonine
  • Thr(α-D-Man(Ac)4)-OH, O-Link Monosaccharide Threonine
  • Thr(α-Man-(1,2)-α-Man)-OH peracetate, O-Link Disaccharide Threonine
  • Thr(β-D-Lac(Ac)7)-OH, O-Link Disaccharide Threonine
  • Thr(β-D-Mal(Ac)7)-OH, O-Link Disaacharide Threonine
We also offer PETYlated Glycoside modifications. Contact us for details.