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Amino Acid Analysis & Quantification for Proteins, Peptides, Biologics, Foods, and Biological Samples

Bio-Synthesis provides high-sensitivity amino acid profiling, amino acid quantification, and protein characterization services using Waters Acquity UPLC AccQ-Tag technology and GLP-guideline analytical workflows.

UPLC AccQ-Tag Analysis Low Microgram Sensitivity Protein & Peptide Characterization Up to 42 Amino Acids & Related Compounds

Amino acid analysis and quantification services for complex biological and pharmaceutical samples

Amino acid analysis is used to determine the amino acid composition, concentration, and identity of proteins, peptides, glycoproteins, biological fluids, pharmaceutical compounds, food products, and nutritional supplements. Bio-Synthesis supports both free amino acid analysis and hydrolysis-based amino acid quantification from proteins and other macromolecules.

Comprehensive Analysis

Quantification of up to 42 amino acids and related compounds.

Fast Turnaround

Results delivered within 5–7 business days for most projects.

Quality & Compliance

GLP-guideline workflows with validated analytical methods.

Expert Support

Experienced scientists available for consultation and project review.

Amino Acid Analysis UPLC Platform

42

Amino acids and related compounds supported

5-7

Business day standard turnaround

UPLC

Waters Acquity AccQ-Tag platform

GLP

Guideline analytical workflows

Amino acid analysis services organized around your sample type and objective

Select from standard hydrolysis and amino acid analysis, biological fluid profiling, food and supplement testing, modified amino acid analysis, or expert data review and consultation.

Service Description Turnaround Price
Standard HCl Hydrolysis + Amino Acid Analysis Quantification of amino acids released from proteins and peptides. Standard HCl hydrolysis may produce limited recovery of cysteine and tryptophan. 5-7 business days $450/sample
Buffer Blank or Modified Amino Acid Standard Analysis Analysis of buffer blanks, unusual matrices, modified amino acid standards, or background amino acid content. 5-7 business days $300/sample
Amino Acid Analysis Data Review & Consultation Expert review of customer-provided amino acid datasets with interpretation, comments, and scientific recommendations. 3-5 business days $400
Amino Acid Hydrolysis Only Acid hydrolysis with acid dried off and hydrolysate returned to the customer for downstream use. 3-5 business days $200/sample

Waters Acquity UPLC AccQ-Tag amino acid analysis

Bio-Synthesis performs amino acid analysis using a Waters Acquity UPLC AAA Analysis system with Binary Solvent Manager, Sample Manager, TUV detector, and Empower software. Pre-column derivatization is performed using AccQ-Tag chemistry, followed by reversed-phase chromatographic separation and detection.

Analytical Workflow

  • Sample hydrolysis or free amino acid preparation
  • AQC pre-column derivatization
  • Reverse-phase UPLC separation
  • Comprehensive analytical reporting

Testing Capabilities

  • All 20 standard amino acids
  • Selected non-standard amino acids
  • Amino sugars and related compounds
  • Low microgram sensitivity

Reporting

  • Quantification results
  • Sample-specific comments
  • Consultation available
  • Confidential data handling

Prepare samples properly for accurate amino acid quantification

Proper sample preparation helps reduce contamination, improve recovery, and support reliable amino acid analysis results. Contact Bio-Synthesis before submission if your samples contain detergents, salts, preservatives, unusual buffers, hazardous materials, or very low concentrations.

Protein & Peptide Samples

  • Recommended amount: 50-200 µg when available
  • Lyophilized samples preferred
  • Provide concentration and purity when known
  • Avoid detergents, salts, and interfering additives

Biological Fluids

  • Plasma, serum, urine, CSF, and related fluids accepted
  • Freeze promptly after collection
  • Avoid repeated freeze-thaw cycles
  • Ship temperature-sensitive samples on dry ice

Food & Supplement Samples

  • Submit powdered or homogenized material when possible
  • Include formulation details when available
  • Identify supplemental free amino acids
  • Contact us for complex or high-fat matrices

Important Hydrolysis Notes

Standard HCl hydrolysis is suitable for many protein and peptide samples, but cysteine and tryptophan may show reduced recovery. If accurate cysteine or tryptophan quantification is required, discuss your project with Bio-Synthesis before submitting samples.

Shipping Recommendations

Use clearly labeled, leak-proof containers. Include absorbent material for liquids and ship biological or temperature-sensitive materials by overnight courier with dry ice when appropriate.

A clear submission process from quote request to final report

Bio-Synthesis reviews each amino acid analysis project before sample submission so the correct method, sample amount, pricing, and turnaround expectations are confirmed in advance.

1

Request a Quote

Send sample type, number of samples, estimated concentration, target analysis, and required turnaround.

2

Confirm Project Details

Bio-Synthesis reviews the project and provides pricing, sample requirements, shipping instructions, and expected timeline.

3

Ship Samples

Submit clearly labeled samples with project reference information and any required sample submission documentation.

4

Receive Report

Receive amino acid quantification results, analytical report, and scientific comments when applicable.

Include With Your Submission

  • Sample names and unique identifiers
  • Sample type and matrix information
  • Estimated concentration or amount
  • Requested service and analysis objective
  • Special handling or storage requirements

Contact Information

Email: info@biosyn.com

For custom projects, unusual amino acids, difficult matrices, modified compounds, or low-abundance samples, contact our scientific support team before shipping.

Contact Technical Support

FAQ

What sample types can be analyzed?
Bio-Synthesis analyzes proteins, peptides, biological fluids, tissue samples, food products, nutritional supplements, pharmaceutical compounds, and selected modified amino acid standards.
Can cysteine and tryptophan be quantified?
Standard HCl hydrolysis may result in reduced recovery of cysteine and tryptophan. If these analytes are critical, discuss alternative approaches before submitting samples.
What is the typical turnaround time?
Most amino acid analysis projects are completed within 5-7 business days after sample receipt and project confirmation.
Do you analyze non-standard amino acids?
Selected non-standard or modified amino acids may be analyzed. Please contact Bio-Synthesis with the analyte name, structure if available, sample matrix, and required quantification needs.
How much sample should I submit?
Requirements depend on sample type, matrix, and expected amino acid concentration. For proteins and peptides, 50-200 ug is recommended when available. Contact us for low-abundance or limited samples.

Related Services

Peptide Synthesis

Custom peptide synthesis services for research and pharmaceutical applications.

Protein Characterization

Analytical testing and characterization of proteins and biologics.

Mass Spectrometry

LC-MS and MALDI mass spectrometry analysis services.

Request amino acid analysis, quantification, or sample review

Submit your sample type, number of samples, target analysis, and turnaround needs. Our scientific team will review your project and recommend the appropriate amino acid testing workflow.
Protein & Peptide Analysis
Biological Fluid Profiling
Food & Supplement Testing
Amino Acid Analysis Services

Need Technical Guidance?

Discuss sample type, hydrolysis needs, cysteine or tryptophan recovery, non-standard amino acids, or biological matrix concerns.

Contact Us →

Ready to Submit?

Confirm project details before shipping samples to ensure proper handling, preparation, pricing, and turnaround.

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Why Choose Bio-Synthesis

Trusted by biotech leaders worldwide for over 45+ years of delivering high quality, fast and scalable synthetic biology solutions.